Circadian rhythm genes CLOCK and PER3 polymorphisms and morning gastric motility in humans.
Circadian rhythm genes CLOCK and PER3 polymorphisms and morning gastric motility in humans.
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DOI：
10.1371/journal.pone.0120009
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Nagai N
中科院分区：
文献类型：
--
作者：
Yamaguchi M;Kotani K;Tsuzaki K;Takagi A;Motokubota N;Komai N;Sakane N;Moritani T;Nagai N
Clock genes regulate circadian rhythm and are involved in various physiological processes, including digestion. We therefore investigated the association between the CLOCK 3111T/C single nucleotide polymorphism and the Period3 (PER3) variable-number tandem-repeat polymorphism (either 4 or 5 repeats 54 nt in length) with morning gastric motility. Lifestyle questionnaires and anthropometric measurements were performed with 173 female volunteers (mean age, 19.4 years). Gastric motility, evaluated by electrogastrography (EGG), blood pressure, and heart rate levels were measured at 8:30 a.m. after an overnight fast. For gastric motility, the spectral powers (% normal power) and dominant frequency (DF, peak of the power spectrum) of the EGG were evaluated. The CLOCK and PER3 polymorphisms were determined by polymerase chain reaction (PCR) restriction fragment length polymorphism analysis. Subjects with the CLOCK C allele (T/C or C/C genotypes: n = 59) showed a significantly lower DF (mean, 2.56 cpm) than those with the T/T genotype (n = 114, 2.81 cpm, P < 0.05). Subjects with the longer PER3 allele (PER3 4/5 or PER3 5/5 genotypes: n = 65) also showed a significantly lower DF (2.55 cpm) than those with the shorter PER3 4/4 genotype (n = 108, 2.83 cpm, P < 0.05). Furthermore, subjects with both the T/C or C/C and PER3 4/5 or PER3 5/5 genotypes showed a significantly lower DF (2.43 cpm, P < 0.05) than subjects with other combinations of the alleles (T/T and PER3 4/4 genotype, T/C or C/C and PER3 4/4 genotypes, and T/T and PER3 4/5 or PER3 5/5 genotypes). These results suggest that minor polymorphisms of the circadian rhythm genes CLOCK and PER3 may be associated with poor morning gastric motility, and may have a combinatorial effect. The present findings may offer a new viewpoint on the role of circadian rhythm genes on the peripheral circadian systems, including the time-keeping function of the gut.
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影响因子：
--
作者：
Marcheva, Biliana;Ramsey, Kathryn M;Peek, Clara B;Affinati, Alison;Maury, Eleonore;Bass, Joseph
通讯作者：
Bass, Joseph
影响因子：
3.7
作者：
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5.6
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Leboyer, M.
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通讯作者：
Archer, Simon N.