A conidiation‐related gene is highly expressed at the resting urediospore stage in Puccinia striiformis f. sp. tritici
A conidiation‐related gene is highly expressed at the resting urediospore stage in Puccinia striiformis f. sp. tritici
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小麦条锈菌 (Puccinia striiformis f.) 的休眠期孢子形成相关基因高度表达。
DOI：
10.1002/jobm.201200059
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发表时间：
2013
影响因子：
3.1
通讯作者：
Z. Kang
中科院分区：
文献类型：
--
作者：
Jun Guo;Y. Duan;Jinshan Zhang;Xuexia Shi;Yue‐Ying Chen;Hong Zhang;Li;Z. Kang
多种真菌无性孢子的产生和萌发在其感染周期中起着至关重要的作用。这些过程是由一组基因调控的，即分生相关基因，参与分生孢子的产生、形态特征和分化。在这项研究中，我们鉴定并鉴定了PsCon1基因，这是第一个在小麦纹状锈菌（Pst）中鉴定到的分生相关基因。序列分析表明，PsCON1具有两个保守的分生作用特异性蛋白6结构域。在5个Pst小种的PsCon1编码区检测到单核苷酸多态性和插入/缺失变异。RT‐PCR结果显示，PsCon1在Pst静息孢子中表达量最高，在萌发和感染后逐渐降低。然而，在312 hpi时，在叶片上形成大量尿孢子的阶段，PsCon1 mRNA的数量急剧增加，但仅为静止尿孢子的0.1倍。亚细胞定位分析表明，PsCon1在稻瘟病菌中异源表达，位于分生孢子细胞质中。结果提示PsCon1可能在Pst孢子的形成或存活中起作用。
The production and germination of asexual spores in a diverse group of fungi play a crucial role in their infection cycles. These processes are regulated by a set of genes, namely, conidiation‐related genes, involved in the production, morphological characteristics, and differentiation of conidia. In this study, we identified and characterized the PsCon1 gene, which is the first conidiation‐related gene identified in Puccinia striiformis f. sp. tritici (Pst). Sequence analysis revealed that PsCON1 has two conserved conidiation‐specific protein 6 domains. Single nucleotide polymorphisms and insertion/deletion variations were detected in the coding region of PsCon1 among five Pst races. Quantitative RT‐PCR assays revealed that PsCon1 was expressed at the highest level in resting urediospores of Pst, and gradually decreased after germination and infection. However, at 312 hpi, at the stage of forming large amounts of urediospores on leaves, the amount of PsCon1 mRNA was sharply increased but only 0.1‐fold that of resting urediospores. Subcellular localization assays indicated PsCon1 heterologously expressed in Fusarium graminearum was located in the cytoplasm of conidia. The results suggest that PsCon1 may play a role in formation or survival of Pst urediospores.
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DOI：
10.1006/fgbi.2001.1264
发表时间：
2001-04
期刊：
Fungal genetics and biology : FG & B
影响因子：
--
作者：
Lori Bailey Shrode;Zachary A. Lewis;Lori D. White;D. Bell-Pedersen;D. Ebbole
通讯作者：
Lori Bailey Shrode;Zachary A. Lewis;Lori D. White;D. Bell-Pedersen;D. Ebbole
DOI：
10.1073/pnas.90.17.8249
发表时间：
1993-09-01
影响因子：
11.1
作者：
LAUTER, FR;YANOFSKY, C
通讯作者：
YANOFSKY, C
粗糙脉孢菌的蓬松基因编码分生孢子发育所需的 Gal4p 型 C6 锌簇蛋白。
影响因子：
3.3
作者：
Bailey,LA;Ebbole,DJ
通讯作者：
Ebbole,DJ
脉孢菌分生孢子基因 con-6 的结构特征和表达分析。
影响因子：
2.7
作者：
White,BT;Yanofsky,C
通讯作者：
Yanofsky,C
rco-3，粗糙脉孢菌中参与葡萄糖转运和分生孢子形成的基因。
影响因子：
3.3
作者：
Madi,L;McBride,SA;Bailey,LA;Ebbole,DJ
通讯作者：
Ebbole,DJ