Comparative analysis of 3D expression patterns of transcription factor genes and digit fate maps in the developing chick wing.
Comparative analysis of 3D expression patterns of transcription factor genes and digit fate maps in the developing chick wing.
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DOI：
10.1371/journal.pone.0018661
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发表时间：
2011-04-22
期刊：
影响因子：
3.7
通讯作者：
Tickle C
中科院分区：
文献类型：
--
作者：
Fisher M;Downie H;Welten MC;Delgado I;Bain A;Planzer T;Sherman A;Sang H;Tickle C
Hoxd 13、Tbx 2、Tbx 3、Sall 1和Sall 3基因是编码发育中鸡翅膀的前后位置值和指定手指身份的候选基因。为了建立一个详细的基因表达模式的细胞谱系，随着时间的推移，产生每个数字，我们比较了三维（3D）的表达模式，这些基因在翅膀的发展，并将它们与数字的命运地图。将使用光学投影断层扫描（OPT）从原位杂交全标本捕获的跨越早期芽到指板形成的阶段21、24和27的3D基因表达数据映射到参考翅芽模型。使用来自GFP鸡胚胎的翅芽组织的移植物来命运映射产生每个手指的翅芽区域;使用OPT捕获移植物的3D图像并映射到相同的模型上。对组合的计算机化数据的计算分析显示，Tbx 2和Tbx 3在所有阶段在第3和第4指祖细胞中表达，与在早期芽中建立的编码稳定的前后位置值一致; Hoxd 13和Sall 1表达更动态，在早期芽中与后趾3和4祖细胞相关，但后来在趾中与前趾2祖细胞相关，板Sox 9在数字缩合中的表达位于由命运映射定义的数字祖细胞的结构域内;数字3缩合表达Hoxd 13和Sall 1，数字4缩合表达Hoxd 13、Tbx 3和较小程度的Tbx 2。Sall 3仅在第24阶段与Sall 1和Hoxd 13一起在数字3祖细胞中瞬时表达;然后从数字板中排除。这些动态的表达模式表明，这些基因可能发挥不同的作用，在手指身份无论是在一起或组合在不同的阶段，包括数字缩合阶段。
Hoxd13, Tbx2, Tbx3, Sall1 and Sall3 genes are candidates for encoding antero-posterior positional values in the developing chick wing and specifying digit identity. In order to build up a detailed profile of gene expression patterns in cell lineages that give rise to each of the digits over time, we compared 3 dimensional (3D) expression patterns of these genes during wing development and related them to digit fate maps. 3D gene expression data at stages 21, 24 and 27 spanning early bud to digital plate formation, captured from in situ hybridisation whole mounts using Optical Projection Tomography (OPT) were mapped to reference wing bud models. Grafts of wing bud tissue from GFP chicken embryos were used to fate map regions of the wing bud giving rise to each digit; 3D images of the grafts were captured using OPT and mapped on to the same models. Computational analysis of the combined computerised data revealed that Tbx2 and Tbx3 are expressed in digit 3 and 4 progenitors at all stages, consistent with encoding stable antero-posterior positional values established in the early bud; Hoxd13 and Sall1 expression is more dynamic, being associated with posterior digit 3 and 4 progenitors in the early bud but later becoming associated with anterior digit 2 progenitors in the digital plate. Sox9 expression in digit condensations lies within domains of digit progenitors defined by fate mapping; digit 3 condensations express Hoxd13 and Sall1, digit 4 condensations Hoxd13, Tbx3 and to a lesser extent Tbx2. Sall3 is only transiently expressed in digit 3 progenitors at stage 24 together with Sall1 and Hoxd13; then becomes excluded from the digital plate. These dynamic patterns of expression suggest that these genes may play different roles in digit identity either together or in combination at different stages including the digit condensation stage.
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影响因子：
2.7
作者：
Fisher, Malcolm E.;Clelland, Allyson K.;Bain, Andrew;Baldock, Richard A.;Murphy, Paula;Downie, Helen;Tickle, Cheryll;Davidson, Duncan R.;Buckland, Richard A.
通讯作者：
Buckland, Richard A.
DOI：
10.1007/bf00326592
发表时间：
1989-01-01
期刊：
ANATOMY AND EMBRYOLOGY
影响因子：
--
作者：
BOWEN, J;HINCHLIFFE, JR;REEVE, AMF
通讯作者：
REEVE, AMF
影响因子：
2.6
作者：
Farrell, ER;Tosh, G;Münsterberg, AE
通讯作者：
Münsterberg, AE
影响因子：
64.8
作者：
IZPISUABELMONTE, JC;TICKLE, C;DUBOULE, D
通讯作者：
DUBOULE, D
影响因子：
56.9
作者：
Dahn, RD;Fallon, JF
通讯作者：
Fallon, JF