An analysis of variability in genome organisation of intracellular calcium release channels across insect orders.
An analysis of variability in genome organisation of intracellular calcium release channels across insect orders.
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跨昆虫阶的细胞内钙释放通道的基因组组织的变异性分析。
DOI：
10.1016/j.gene.2018.05.075
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发表时间：
2018-09-05
期刊：
影响因子：
3.5
通讯作者：
Davies TGE
中科院分区：
文献类型：
--
作者：
Troczka BJ;Richardson E;Homem RA;Davies TGE
利用公开可用的基因组数据，结合RT-PCR验证，我们探索了昆虫类中两个主要离子通道的结构基因组变异。我们人工筛选了26种昆虫的ryanodine受体（RyR）和肌醇1,4,5-三磷酸受体（IP3R） orf及其相应的基因组结构。我们发现，尽管RyRs（约75%）和IP3Rs（约67%）的蛋白质同源性很高，但不同昆虫目之间基因结构的整体复杂性差异很大，双翅目中基因最简单（内含子最少），鳞翅目中基因最复杂。内含子保守模式分析表明，大多数保守的内含子位于通道的5 '端附近和RyR中高度保守的互斥剪接位点附近。在这两个通道中，ip3r似乎有一个不太好的保守组织，在昆虫目之间有更多的独特内含子。我们通过实验验证了两个人工筛选的IP3Rs orf，并在桃蚜中确认了一个非典型（3799aa） IP3R受体，该受体比先前报道的IP3Rs大约1000个氨基酸。绘制了26种昆虫的RyR和IP3R基因结构图谱，不同昆虫目之间RyR和IP3R基因结构的总体复杂性差异较大。在蚜虫中鉴定并实验验证了一个非典型的大IP3R受体
Using publicly available genomic data, combined with RT-PCR validation, we explore structural genomic variation for two major ion channels across insect classes. We have manually curated ryanodine receptor (RyR) and inositol 1,4,5-trisphosphate receptor (IP3R) ORFs and their corresponding genomic structures from 26 different insects covering major insect orders. We found that, despite high protein identity for both RyRs (>75%) and IP3Rs (~67%), the overall complexity of the gene structure varies greatly between different insect orders with the simplest genes (fewest introns) found in Diptera and the most complex in Lepidoptera. Analysis of intron conservation patterns indicated that the majority of conserved introns are found close to the 5′ end of the channels and in RyR around the highly conserved mutually exclusive splice site. Of the two channels the IP3Rs appear to have a less well conserved organisation with a greater overall number of unique introns seen between insect orders. We experimentally validated two of the manually curated ORFs for IP3Rs and confirmed an atypical (3799aa) IP3R receptor in Myzus persicae, which is approximately 1000 amino acids larger than previously reported for IP3Rs. Mapped the genomic structures of RyR and IP3R from 26 different insect species Overall complexity of RyR and IP3R gene structure varies greatly between different insect orders. Identified and experimentally validated an atypically large IP3R receptor in aphid species
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影响因子：
4.9
作者：
Berger M;Puinean AM;Randall E;Zimmer CT;Silva WM;Bielza P;Field LM;Hughes D;Mellor I;Hassani-Pak K;Siqueira HA;Williamson MS;Bass C
通讯作者：
Bass C
影响因子：
12.3
作者：
Keeling CI;Yuen MM;Liao NY;Docking TR;Chan SK;Taylor GA;Palmquist DL;Jackman SD;Nguyen A;Li M;Henderson H;Janes JK;Zhao Y;Pandoh P;Moore R;Sperling FA;Huber DP;Birol I;Jones SJ;Bohlmann J
通讯作者：
Bohlmann J
影响因子：
56.9
作者：
Hoskins, Roger A.;Carlson, Joseph W.;Celniker, Susan E.
通讯作者：
Celniker, Susan E.
影响因子：
4.4
作者：
Kakumani, Pavan Kumar;Malhotra, Pawan;Bhatnagar, Raj K.
通讯作者：
Bhatnagar, Raj K.
影响因子：
3.3
作者：
Cong Q;Shen J;Warren AD;Borek D;Otwinowski Z;Grishin NV
通讯作者：
Grishin NV