Potential-Driven Electrochemical Clearing of Ex Vivo Alkaline Corneal Injuries.
Potential-Driven Electrochemical Clearing of Ex Vivo Alkaline Corneal Injuries.
复制标题
DOI：
10.1167/tvst.11.1.32
复制
发表时间：
2022-01-03
影响因子：
3
通讯作者：
Wong BJF
中科院分区：
文献类型：
--
作者：
Dilley KK;Borden PA;Qu Y;Heidari AE;Prasad KR;Li Y;Sun CH;Chen Z;Kim S;Hill MG;Wong BJF
角膜化学损伤（CCI）通过使角膜混浊而使视力模糊;然而，目前的治疗可能无法完全恢复清晰度。在这里，我们研究了电位驱动的电化学治疗（P-ECT），以恢复清晰度后，碱性CCI在离体兔角膜和检查胶原纤维取向的变化，使用二次谐波发生（SHG）。将NaOH应用于完整新西兰白色家兔眼球的角膜。在混浊角膜上进行P-ECT，同时进行光学相干断层扫描（OCT）成像（每秒35帧）。SHG成像评价了NaOH应用前和P-ECT后的胶原纤维结构。用水灌溉作为对照。P-ECT在NaOH暴露后恢复局部光学清晰度。OCT成像显示NaOH损伤的进展和真实的清晰度的恢复。SHG z-堆叠图像的分析显示胶原原纤维取向在对照、NaOH损伤和P-ECT后角膜之间是相似的。NaOH损伤的角膜用水冲洗（15分钟），未显示透明性恢复。P-ECT可能是纠正碱性CCI的一种手段。胶原原纤维取向在NaOH暴露或P-ECT后没有改变，表明没有发生不可逆的基质水平纤维变化。需要进一步的研究来确定角膜透明化的机制，并确定最佳的电剂量学参数和电极设计。我们的研究结果表明，P-ECT是一种潜在的有效，低成本的治疗碱性CCI。
Corneal chemical injuries (CCI) obscure vision by opacifying the cornea; however, current treatments may not fully restore clarity. Here, we investigated potential-driven electrochemical treatment (P-ECT) to restore clarity after alkaline-based CCI in ex vivo rabbit corneas and examined collagen fiber orientation changes using second harmonic generation (SHG). NaOH was applied to the corneas of intact New Zealand white rabbit globes. P-ECT was performed on the opacified cornea while optical coherence tomography (OCT) imaging (∼35 frames per second) was simultaneously performed. SHG imaging evaluated collagen fiber structure before NaOH application and after P-ECT. Irrigation with water served as a control. P-ECT restored local optical clarity after NaOH exposure. OCT imaging shows both progression of NaOH injury and the restoration of clarity in real time. Analysis of SHG z-stack images show that collagen fibril orientation is similar between control, NaOH-damaged, and post-P-ECT corneas. NaOH-injured corneas flushed with water (15 minutes) show no restoration of clarity. P-ECT may be a means to correct alkaline CCI. Collagen fibril orientation does not change after NaOH exposure or P-ECT, suggesting that no irreversible matrix level fiber changes occur. Further studies are required to determine the mechanism for corneal clearing and to ascertain the optimal electrical dosimetry parameters and electrode designs. Our findings suggest that P-ECT is a potentially effective, low-cost treatment for alkaline CCI.
登录
查看更多内容
影响因子：
--
作者：
Oliaei, Sepehr;Manuel, Cyrus;Wong, Brian J. F.
通讯作者：
Wong, Brian J. F.
影响因子：
4.6
作者：
Pham, Tiffany T.;Hong, Ellen M.;Wong, Brian J. F.
通讯作者：
Wong, Brian J. F.
DOI：
10.1002/lary.25237
发表时间：
2015-09
期刊：
The Laryngoscope
影响因子：
--
作者：
Badran KW;Manuel CT;Loy AC;Conderman C;Yau YY;Lin J;Tjoa T;Su E;Protsenko D;Wong BJ
通讯作者：
Wong BJ
影响因子：
2.6
作者：
Badran, Karam;Manuel, Cyrus;Wong, Brian J. F.
通讯作者：
Wong, Brian J. F.
影响因子：
4.1
作者：
Mashima, Y;Kawai, M;Yamada, M
通讯作者：
Yamada, M