Alterations in composition and diversity of the intestinal microbiota in patients with diarrhea-predominant irritable bowel syndrome.
Alterations in composition and diversity of the intestinal microbiota in patients with diarrhea-predominant irritable bowel syndrome.
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DOI：
10.1111/j.1365-2982.2012.01891.x
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发表时间：
2012-06
影响因子：
3.5
通讯作者：
Ringel Y
中科院分区：
文献类型：
--
作者：
Carroll IM;Ringel-Kulka T;Siddle JP;Ringel Y
肠道微生物群与肠易激综合征（IBS）的病理生理学有关。由于用于表征肠道微生物群的技术的可变分辨率以及IBS的异质性，IBS肠道微生物群的定义的改变是不一致的。我们使用一种技术分析了一个确定的IBS患者亚组（以大肠杆菌为主的IBS，D-IBS）的肠道微生物群组成，该技术为复杂的微生物群落提供了最深入的表征。从23名D-IBS患者和23名健康对照（HC）中分离粪便DNA。从所有样品中扩增16 S rRNA基因的可变区V1-V3和V6。使用454高通量测序对PCR产物进行测序。使用微生物生态学定量洞察管道确定并比较了D-IBS和HC之间微生物群落的组成、多样性和丰富度。细菌群对肠道微生物群组成的贡献在D-IBS和HC之间不同。与HC相比，D-IBS患者具有显著更高水平的肠杆菌科（p = 0.03）和更低水平的粪杆菌属（p = 0.04）。β-多样性值表明HC患者的UniFrac距离水平显著低于D-IBS患者。D-IBS患者16 SrRNA序列丰富度显著降低（p < 0.04）。我们的16 S rRNA序列数据证明了D-IBS中的社区水平的生态失调。D-IBS中肠道微生物群组成的改变与有害细菌群的显著增加和有益细菌群的减少以及微生物丰富度的减少有关。
The intestinal microbiota has been implicated in the pathophysiology of Irritable Bowel Syndrome (IBS). Due to the variable resolutions of techniques used to characterize the intestinal microbiota, and the heterogeneity of IBS, the defined alterations of the IBS intestinal microbiota are inconsistent. We analyzed the composition of the intestinal microbiota in a defined subgroup of IBS patients (diarrhea-predominant IBS, D-IBS) using a technique that provides the deepest characterization available for complex microbial communities. Fecal DNA was isolated from 23 D-IBS patients and 23 healthy controls (HC). Variable regions V1-V3 and V6 of the 16S rRNA gene were amplified from all samples. PCR products were sequenced using 454 high throughput sequencing. The composition, diversity and richness of microbial communities were determined and compared between D-IBS and HC using the Quantitative Insights Into Microbial Ecology pipeline. The contribution of bacterial groups to the composition of the intestinal microbiota differed between D-IBS and HC. D-IBS patients had significantly higher levels of Enterobacteriaceae (p = 0.03), and lower levels of Fecalibacterium genera (p = 0.04) compared to HC. β-diversity values demonstrated significantly lower levels of UniFrac distances in HC compared to D-IBS patients. The richness of 16S rRNA sequences was significantly decreased in D-IBS patients (p < 0.04). Our 16S rRNA sequence data demonstrates a community-level dysbiosis in D-IBS. The altered composition of the intestinal microbiota in D-IBS is associated with significant increases in detrimental and decreases in beneficial bacterial groups, and a reduction in microbial richness.
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DOI：
10.1073/pnas.0807920105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Fierer, Noah;Hamady, Micah;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
3.7
作者：
Andersson, Anders F.;Lindberg, Mathilda;Jakobsson, Hedvig;Backhed, Fredrik;Nyren, Pal;Engstrand, Lars
通讯作者：
Engstrand, Lars
影响因子：
7.6
作者：
Hungin, APS;Chang, L;Barghout, V
通讯作者：
Barghout, V
DOI：
10.1093/bioinformatics/btp636
发表时间：
2010-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Caporaso JG;Bittinger K;Bushman FD;DeSantis TZ;Andersen GL;Knight R
通讯作者：
Knight R
影响因子：
3.1
作者：
Codling, Caroline;O'Mahony, Liam;Marchesi, Julian R.
通讯作者：
Marchesi, Julian R.