Microfluidic cell sorter with integrated piezoelectric actuator.
Microfluidic cell sorter with integrated piezoelectric actuator.
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DOI：
10.1007/s10544-009-9341-5
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发表时间：
2009-12
影响因子：
2.8
通讯作者：
Lo, Yu-Hwa
中科院分区：
文献类型：
--
作者：
Chen, Chun H.;Cho, Sung Hwan;Tsai, Frank;Erten, Ahmet;Lo, Yu-Hwa
我们展示了一个低功耗（<0.1 mW），低电压（<10 Vp-p）的片上压电致动微分选器，可以偏转单粒子和细胞在高速。在流中具有若丹明的情况下，可以在高致动频率（~1.7 kHz）下可视化通道之间的流切换。通过输入电压的大小和波形可以精确地控制单元偏转的大小。模拟和实验结果都表明，由瞬时流体位移施加在悬浮颗粒/细胞上的拖曳力可以以受控方式改变任何尺寸、形状和密度的颗粒/细胞的轨迹。开环E.大肠杆菌细胞偏转实验表明，分选机制可以产生至少330个细胞/s的吞吐量，具有显着更高的吞吐量的优化设计的承诺。为了实现闭环分选操作，开发了荧光检测、实时信号处理和现场可编程门阵列（FPGA）实现的控制算法，以执行荧光珠的自动分选。初步结果表明，无错误的排序，排序效率约为70%。由于压电致动器具有0.1-1 ms的固有响应时间，并且分选可以在高流速（颗粒速度约为1-10 cm/s）下进行，因此系统可以实现> 1，000个颗粒/s的高纯度吞吐量。
We demonstrate a low-power (<0.1 mW), low-voltage (<10 Vp-p) on-chip piezoelectrically actuated micro-sorter that can deflect single particles and cells at high-speed. With rhodamine in the stream, switching of flow between channels can be visualized at high actuation frequency (~1.7 kHz). The magnitude of the cell deflection can be precisely controlled by the magnitude and waveform of input voltage. Both simulation and experimental results indicate that the drag force imposed on the suspended particle/cell by the instantaneous fluid displacement can alter the trajectory of the particle/cell of any size, shape, and density of interest in a controlled manner. The open-loop E. Coli cell deflection experiment demonstrates that the sorting mechanism can produce a throughput of at least 330 cells/s, with a promise of a significantly higher throughput for an optimized design. To achieve close-loop sorting operation, fluorescence detection, real-time signal processing, and field-programmable-gate-array (FPGA) implementation of the control algorithms were developed to perform automated sorting of fluorescent beads. The preliminary results show error-free sorting at a sorting efficiency of ~70%. Since the piezoelectric actuator has an intrinsic response time of 0.1–1 ms and the sorting can be performed under high flowrate (particle speed of ~1–10 cm/s), the system can achieve a throughput of >1,000 particles/s with high purity.
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影响因子：
46.9
作者：
Meredith, GD;Sims, CE;Allbritton, NL
通讯作者：
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影响因子：
6.1
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作者：
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通讯作者：
O'Brien, P