Extensive and intimate association of the cytoskeleton with forming silica in diatoms: control over patterning on the meso- and micro-scale.
Extensive and intimate association of the cytoskeleton with forming silica in diatoms: control over patterning on the meso- and micro-scale.
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DOI：
10.1371/journal.pone.0014300
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发表时间：
2010-12-10
期刊：
影响因子：
3.7
通讯作者：
Hildebrand M
中科院分区：
文献类型：
--
作者：
Tesson B;Hildebrand M
硅藻细胞壁，称为硅藻壳，主要由二氧化硅制成，在许多情况下具有高度有序的纳米和微米尺度特征。硅藻壳在细胞内被构建在一个特殊的隔室，二氧化硅沉积囊泡，或SDV。分子，如蛋白质（silaffins和silacidins）和长链多胺已被分离的二氧化硅和参与控制的二氧化硅聚合。然而，我们仍然无法解释或在体外重现硅藻形成的结构的复杂性。在这项研究中，使用荧光显微镜，扫描电子显微镜和原子力显微镜，我们能够比较和关联的微管和微丝与二氧化硅结构中形成的dilemma结构的硅藻物种。二氧化硅结构和肌动蛋白之间的高度相关性表明，肌动蛋白是一个主要的元素在控制的二氧化硅形态发生在中，微观尺度。微管似乎参与了空间定位的中尺度和加强SDV。这些结果揭示了自上而下的控制的重要性，并在硅藻壁的形成过程中的SDV的定位和打开一个新的视角的硅藻壳图案化的机制的研究，以及为理解控制膜动力学的细胞骨架。
The diatom cell wall, called the frustule, is predominantly made out of silica, in many cases with highly ordered nano- and micro-scale features. Frustules are built intracellularly inside a special compartment, the silica deposition vesicle, or SDV. Molecules such as proteins (silaffins and silacidins) and long chain polyamines have been isolated from the silica and shown to be involved in the control of the silica polymerization. However, we are still unable to explain or reproduce in vitro the complexity of structures formed by diatoms. In this study, using fluorescence microscopy, scanning electron microscopy, and atomic force microscopy, we were able to compare and correlate microtubules and microfilaments with silica structure formed in diversely structured diatom species. The high degree of correlation between silica structure and actin indicates that actin is a major element in the control of the silica morphogenesis at the meso and microscale. Microtubules appear to be involved in the spatial positioning on the mesoscale and strengthening of the SDV. These results reveal the importance of top down control over positioning of and within the SDV during diatom wall formation and open a new perspective for the study of the mechanism of frustule patterning as well as for the understanding of the control of membrane dynamics by the cytoskeleton.
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影响因子：
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