Polyhedra self-assembled from DNA tripods and characterized with 3D DNA-PAINT.

Polyhedra self-assembled from DNA tripods and characterized with 3D DNA-PAINT.
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DOI:
10.1126/science.1250944
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发表时间:
2014-04-04
期刊:
Science (New York, N.Y.)
影响因子:
--
通讯作者:
Yin P
Yin P
中科院分区:
其他
文献类型:
--
作者:
Iinuma R;Ke Y;Jungmann R;Schlichthaerle T;Woehrstein JB;Yin P

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DNA自组装产生了多种合成的三维多面体。这些结构通常具有不大于5兆道尔顿的分子量。我们报告了一种简单、通用的线框 DNA 多面体一步自组装策略,该多面体比大多数以前的结构更大。具有精确控制角度和臂长的刚性三臂连接 DNA 折纸图块图案用于多面体的分层组装。我们实验性地构建了边宽为 100 纳米的四面体(20 兆道尔顿)、三棱柱(30 兆道尔顿)、立方体(40 兆道尔顿)、五角柱(50 兆道尔顿)和六角柱(60 兆道尔顿)。通过透射电子显微镜和溶液中单分子的三维 DNA-PAINT 超分辨率荧光显微镜观察结构。
DNA self-assembly has produced diverse synthetic three-dimensional polyhedra. These structures typically have a molecular weight no greater than 5 megadaltons. We report a simple, general strategy for one-step self-assembly of wireframe DNA polyhedra that are more massive than most previous structures. A stiff three-arm-junction DNA origami tile motif with precisely controlled angles and arm lengths was used for hierarchical assembly of polyhedra. We experimentally constructed a tetrahedron (20 megadaltons), a triangular prism (30 megadaltons), a cube (40 megadaltons), a pentagonal prism (50 megadaltons), and a hexagonal prism (60 megadaltons) with edge widths of 100 nanometers. The structures were visualized by means of transmission electron microscopy and three-dimensional DNA-PAINT super-resolution fluorescent microscopy of single molecules in solution.
DOI: 10.1021/nl901165f
发表时间: 2009-06-01
期刊: NANO LETTERS
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