Polyhedra self-assembled from DNA tripods and characterized with 3D DNA-PAINT.
Polyhedra self-assembled from DNA tripods and characterized with 3D DNA-PAINT.
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DOI:
10.1126/science.1250944
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发表时间:
2014-04-04
期刊:
影响因子:
--
通讯作者:
Yin P
中科院分区:
文献类型:
--
作者:
Iinuma R;Ke Y;Jungmann R;Schlichthaerle T;Woehrstein JB;Yin P
DNA self-assembly has produced diverse synthetic three-dimensional polyhedra. These structures typically have a molecular weight no greater than 5 megadaltons. We report a simple, general strategy for one-step self-assembly of wireframe DNA polyhedra that are more massive than most previous structures. A stiff three-arm-junction DNA origami tile motif with precisely controlled angles and arm lengths was used for hierarchical assembly of polyhedra. We experimentally constructed a tetrahedron (20 megadaltons), a triangular prism (30 megadaltons), a cube (40 megadaltons), a pentagonal prism (50 megadaltons), and a hexagonal prism (60 megadaltons) with edge widths of 100 nanometers. The structures were visualized by means of transmission electron microscopy and three-dimensional DNA-PAINT super-resolution fluorescent microscopy of single molecules in solution.
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发表时间:
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期刊:
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影响因子:
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通讯作者:
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