Evolution of Tembusu Virus in Ducks, Chickens, Geese, Sparrows, and Mosquitoes in Northern China.
Evolution of Tembusu Virus in Ducks, Chickens, Geese, Sparrows, and Mosquitoes in Northern China.
复制标题
中国北方鸭、鸡、鹅、麻雀和蚊子中天布苏病毒的进化
DOI：
10.3390/v10090485
复制
发表时间：
2018-09-10
期刊：
影响因子：
--
通讯作者：
Diao Y
中科院分区：
文献类型：
--
作者：
Yu G;Lin Y;Tang Y;Diao Y
坦布苏病毒(Tembusu Virus，TMUV)是一种主要感染鸭和鹅的禽类传染性病原体，可引起高烧、食欲不振、生长迟缓、神经系统症状、严重的跌鸭综合征，甚至死亡。2011年至2017年，我们在中国北部地区进行了TMUV流行病学调查，从鸭、鸡、鹅、麻雀和蚊子中分离到11株TMUV。核苷酸和氨基酸同源性分别为85.8-99.8%和92.5-99.68%，分别低于E株(86.7-99.9%和96.5-99.9%)、NS3株(87.6-99.9%和98.2-99.8%)和NS5株(86.5-99.9%和97.8-99.9%)。对病毒蛋白三级结构的预测表明，11株病毒中有4株的NS1在180TAV182处有蛋白质结构突变，将随机卷曲改变为α螺旋。从NTTD到NITD，11株菌株中有6株存在糖基化位点突变。此外，流行病学数据表明，该病已在中国所在省份的一半地区流行(34个省份中有17个)。我们的发现首次将NS1蛋白确定为遗传进化的潜在高变区。此外，该病毒的地域范围已经扩大，需要严格的生物安全措施或多价疫苗来控制其传播。
Tembusu virus (TMUV) is a contagious pathogen from fowl that mainly infects ducks and geese, causing symptoms of high fever, loss of appetite, retarded growth, neurological symptoms, severe duck-drop syndrome, and even death. During an epidemiological investigation of TMUV in Northern China, we isolated 11 TMUV strains from ducks, chickens, geese, sparrows, and mosquitoes (2011–2017). Phylogenetic analysis of the open-reading frames of genes revealed that these strains clustered into Chinese strains II. The nucleotide and amino acid homologies of NS1 of the strains ranged between 85.8–99.8% and 92.5–99.68%, respectively, which were lower than those of E (86.7–99.9% and 96.5–99.9%, respectively), NS3 (87.6–99.9% and 98.2–99.8%, respectively), and NS5 (86.5–99.9% and 97.8–99.9%, respectively). Predictions of the tertiary structure of the viral proteins indicated that NS1 in 4 of 11 strains had a protein structure mutation at 180TAV182 that changed a random crimp into an alpha helix. The protein of 6 of 11 strains had a glycosylation site mutation from NTTD to NITD. Furthermore, epidemiological data suggested that TMUV has been circulating in half of China’s provinces (17 of 34). Our findings, for the first time, have identified the NS1 protein as a potential hypervariable region for genetic evolution. Additionally, the territorial scope of the virus has expanded, requiring strict bio-security measures or a multivalent vaccine to control its spread.
登录
查看更多内容
影响因子：
3.7
作者：
Yu K;Sheng ZZ;Huang B;Ma X;Li Y;Yuan X;Qin Z;Wang D;Chakravarty S;Li F;Song M;Sun H
通讯作者：
Sun H
影响因子：
4.8
作者：
Li S;Li X;Zhang L;Wang Y;Yu X;Tian K;Su W;Han B;Su J
通讯作者：
Su J
新出现的中国鸭落蛋综合症黄病毒的基因组和抗原特征：与坦布苏病毒和实兆远病毒的基因组比较
影响因子：
3.8
作者：
Liu, Peipei;Lu, Hao;Gao, George F.
通讯作者：
Gao, George F.
影响因子：
2.8
作者：
Homonnay, Zalan Gabor;Kovacs, Edit Walkone;Palya, Vilmos
通讯作者：
Palya, Vilmos
鸭天宝苏病毒脑内接种对昆明小鼠表现出致病性
影响因子：
5.2
作者：
Ti J;Zhang M;Li Z;Li X;Diao Y
通讯作者：
Diao Y