Profile of dorsal root ganglion neurons: study of oxytocin expression.
Profile of dorsal root ganglion neurons: study of oxytocin expression.
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DOI：
10.1186/s13041-022-00927-6
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发表时间：
2022-05-09
期刊：
影响因子：
3.6
通讯作者：
中科院分区：
文献类型：
--
作者：
尽管背根神经节（DRG）神经元迄今为止已根据其纤维的差异（Aβ、Aδ和C）进行分类，但这种分类应根据基因表达模式进一步细分。由于催产素（oxytocin，OXT）在DRG神经元中起局部作用，因此我们将重点放在OXT及其相关受体上。采用实时荧光定量PCR技术检测单个DRG神经元中OXT、OXT受体（OXTR）、加压素V1 a受体（V1 aR）、瞬时受体电位阳离子通道亚家族V成员1（TRPV 1）和压电型机械敏感离子通道组分2（Piezo 2）的mRNA水平，并进行聚类分析。根据基因表达模式，DRG神经元可分为4个簇：簇1主要以Piezo 2为特征，簇2以TRPV 1为特征，簇4以OXTR为特征，簇3中的神经元不表达任何靶基因。OXT表达神经元的细胞体直径在簇1中比在簇2中显著更大。这些结果表明，具有小细胞体（簇2）和大细胞体（簇1）的表达OXT的DRG神经元可能分别对应于C纤维神经元和Aβ纤维神经元。此外，表达OXT的神经元不仅含有TRPV 1，而且还含有Piezo 2，这表明OXT可以通过机械刺激而释放，而不管伤害感受如何。因此，机械感受和伤害感受本身可诱导DRG中OXT的自分泌/旁分泌功能，有助于缓解疼痛。
Although dorsal root ganglion (DRG) neurons have been so far classified according to the difference in their fibers (Aβ, Aδ, and C), this classification should be further subdivided according to gene expression patterns. We focused on oxytocin (OXT) and its related receptors, because OXT plays a local role in DRG neurons. We measured the mRNA levels of OXT, OXT receptor (OXTR), vasopressin V1a receptor (V1aR), transient receptor potential cation channel subfamily V member 1 (TRPV1), and piezo-type mechanosensitive ion channel component 2 (Piezo2) in single DRG neurons by using real-time PCR, and then performed a cluster analysis. According to the gene expression patterns, DRG neurons were classified into 4 clusters: Cluster 1 was characterized mainly by Piezo2, Cluster 2 by TRPV1, Cluster 4 by OXTR, and neurons in Cluster 3 did not express any of the target genes. The cell body diameter of OXT-expressing neurons was significantly larger in Cluster 1 than in Cluster 2. These results suggest that OXT-expressing DRG neurons with small cell bodies (Cluster 2) and large cell bodies (Cluster 1) probably correspond to C-fiber neurons and Aβ-fiber neurons, respectively. Furthermore, the OXT-expressing neurons contained not only TRPV1 but also Piezo2, suggesting that OXT may be released by mechanical stimulation regardless of nociception. Thus, mechanoreception and nociception themselves may induce the autocrine/paracrine function of OXT in the DRG, contributing to alleviation of pain.
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影响因子：
1.5
作者：
Ito, Etsuro;Shima, Rei;Yoshioka, Tohru
通讯作者：
Yoshioka, Tohru
DOI：
10.2142/biophysics.11.33
发表时间：
2015
期刊：
Biophysics (Nagoya-shi, Japan)
影响因子：
--
作者：
Ito E;Ikemoto Y;Yoshioka T
通讯作者：
Yoshioka T
影响因子：
3.3
作者：
Saito, Norio;Shima, Rei;Yoshioka, Tohru
通讯作者：
Yoshioka, Tohru
影响因子：
56.9
作者：
MELZACK, R;WALL, PD
通讯作者：
WALL, PD
影响因子：
7.4
作者：
Mendell LM
通讯作者：
Mendell LM