Immobilizing enzymes onto electrode arrays by hydrogel photolithography to fabricate multi-analyte electrochemical biosensors.
Immobilizing enzymes onto electrode arrays by hydrogel photolithography to fabricate multi-analyte electrochemical biosensors.
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DOI：
10.1021/am9007819
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发表时间：
2010-03
影响因子：
9.5
通讯作者：
Revzin, Alexander
中科院分区：
文献类型：
--
作者：
Yan, Jun;Pedrosa, Valber A.;Simonian, Aleksandr L.;Revzin, Alexander
本文介绍了一种生物材料微加工方法接口功能的生物分子（酶）与电极阵列。采用聚乙二醇（PEG）水凝胶修饰法将葡萄糖氧化酶（GOX）和乳酸氧化酶（LOX）与金电极阵列进行了组装。在该过程中，将含有酶分子以及氧化还原物质（乙烯基二茂铁）的PEG二丙烯酸酯（DA）基预聚物旋涂、配准并UV交联在金电极阵列的顶部。结果，在300 µm直径的金电极上制造了携带酶的圆形水凝胶结构（直径600 µm）。重要的是，当与多个掩模一起使用时，水凝胶光刻允许我们将GOX和LOX分子固定在同一电极阵列内的相邻电极上。循环伏安法和电流法被用来表征生物传感器电极阵列。生物传感器阵列的响应对于高达20 mM的葡萄糖是线性的，灵敏度为0.9 µA cm−2 mM−1，对于10 mM的乳酸盐是线性的，灵敏度为1.1 µA cm−2 mM−1。重要的是，证明了从同一电极阵列同时检测葡萄糖和乳酸。本文所述的生物传感器的生物和电气组件集成的一种新的策略提供了空间上解决和注册不同的生物识别元件与微型电极阵列的各个成员的灵活性。我们特别感兴趣的是这些微型电极在活细胞附近的代谢物通量的实时监测的未来应用。
This paper describes a biomaterial microfabrication approach for interfacing functional biomolecules (enzymes) with electrode arrays. Poly (ethylene glycol) (PEG) hydrogel photopatterning was employed to integrate gold electrode arrays with enzymes - glucose oxidase (GOX) and lactate oxidase (LOX). In this process, PEG diacrylate (DA)-based prepolymer containing enzyme molecules as well as redox species (vinylferrocene) was spin-coated, registered and UV cross-linked on top of an array of gold electrodes. As a result, enzyme-carrying circular hydrogel structures (600 µm diameter) were fabricated on top of 300 µm diameter gold electrodes. Importantly, when used with multiple masks, hydrogel photolithography allowed us to immobilize GOX and LOX molecules on adjacent electrodes within the same electrode array. Cyclic voltammetry and amperometry were used to characterize biosensor electrode arrays. The response of the biosensor array was linear for up to 20 mM glucose with sensitivity of 0.9 µA cm−2 mM−1 and 10 mM lactate with sensitivity of 1.1 µA cm−2 mM−1. Importantly, simultaneous detection of glucose and lactate from the same electrode array was demonstrated. A novel strategy for integrating biological and electrical components of a biosensor described in this paper provides the flexibility to spatially resolve and register different biorecognition elements with individual members of a miniature electrode array. Of particular interest to us are future applications of these miniature electrodes for real-time monitoring of metabolite fluxes in the vicinity of living cells.
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DOI：
10.1021/la803635r
发表时间：
2009-04-09
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Lee JY;Shah SS;Yan J;Howland MC;Parikh AN;Pan T;Revzin A
通讯作者：
Revzin A
影响因子：
6.2
作者：
Jimenez, C;Bartrol, J;KoudelkaHep, M
通讯作者：
KoudelkaHep, M
DOI：
10.1007/bf00321250
发表时间：
1993-06-01
期刊：
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY
影响因子：
--
作者：
LINDNER, E;COSOFRET, VV;BUCK, RP
通讯作者：
BUCK, RP
影响因子：
3.9
作者：
Merchant, Stephen A.;Tran, Tu O.;Schmidtke, David W.
通讯作者：
Schmidtke, David W.
影响因子：
12.6
作者：
Moser, I;Jobst, G;Urban, GA
通讯作者：
Urban, GA