An induced hypersensitive-like response limits expression of foreign peptides via a recombinant TMV-based vector in a susceptible tobacco.
An induced hypersensitive-like response limits expression of foreign peptides via a recombinant TMV-based vector in a susceptible tobacco.
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诱导的类过敏反应通过基于 TMV 的重组载体在易感烟草中限制外源肽的表达
DOI：
10.1371/journal.pone.0015087
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发表时间：
2010-11-29
期刊：
影响因子：
3.7
通讯作者：
Xu Z
中科院分区：
文献类型：
--
作者：
Li M;Li P;Song R;Xu Z
背景利用基于烟草花叶病毒(TMV)的载体，食品月病病毒(FMDV)VP1蛋白的外源表位可以在TMV外壳蛋白(CP)的C末端附近融合，并在感病烟草中高效表达。此前，我们已经证明，显示口蹄疫病毒VP1表位的重组TMV疫苗可以在豚鼠和猪身上产生保护作用，对抗口蹄疫病毒的挑战。最近，一些重组TMV，如含有口蹄疫病毒VP1表位FN20的TMVFN20，被发现在感病烟草上引起局部坏死病(LNL)。这种超敏反应(HLR)阻断了烟草中重组TMVFN20的扩增，限制了针对口蹄疫病毒的重组TMV疫苗的应用。方法/主要发现在易感的Samsunnn中，我们研究了HLR的分子机制。组织化学染色分析表明，这些LNL与接种野生型(Wt)TMV的抗病烟草SamsunNN中诱导的LNL相似。重组CP亚基与HLR特异性相关。有趣的是，在缺乏N/N‘-基因的Samsunnn中，重组TMV在25℃和33℃都能诱导这种HLR，而wt TMV通过N/N’-基因途径在抗性烟草植株上诱导的过敏反应(HR)只能在允许的温度下(低于30℃)诱导。此外，我们首次报道了烟草中一些与防御反应(DR)相关的基因在HLR过程中转录上调。结论与HR不同，HLR在感病烟草中是通过N/N‘基因非依赖的途径诱导的。HLR的诱导与重组CP亚基的表达和DR相关基因的上调有关。
Background By using tobacco mosaic virus (TMV)-based vectors, foreign epitopes of the VP1 protein from food-and-month disease virus (FMDV) could be fused near to the C-terminus of the TMV coat protein (CP) and expressed at high levels in susceptible tobacco plants. Previously, we have shown that the recombinant TMV vaccines displaying FMDV VP1 epitopes could generate protection in guinea pigs and swine against the FMDV challenge. Recently, some recombinant TMV, such as TMVFN20 that contains an epitope FN20 from the FMDV VP1, were found to induce local necrotic lesions (LNL) on the inoculated leaves of a susceptible tobacco, Nicotiana tabacum Samsun nn. This hypersensitive-like response (HLR) blocked amplification of recombinant TMVFN20 in tobacco and limited the utility of recombinant TMV vaccines against FMDV. Methodology/Principal Findings Here we investigate the molecular mechanism of the HLR in the susceptible Samsun nn. Histochemical staining analyses show that these LNL are similar to those induced in a resistant tobacco Samsun NN inoculated with wild type (wt) TMV. The recombinant CP subunits are specifically related to the HLR. Interestingly, this HLR in Samsun nn (lacking the N/N′-gene) was able to be induced by the recombinant TMV at both 25°C and 33°C, whereas the hypersensitive response (HR) in the resistant tobacco plants induced by wt TMV through the N/N′-gene pathways only at a permissive temperature (below 30°C). Furthermore, we reported for the first time that some of defense response (DR)-related genes in tobacco were transcriptionally upregulated during HLR. Conclusions Unlike HR, HLR is induced in the susceptible tobacco through N/N′-gene independent pathways. Induction of the HLR is associated with the expression of the recombinant CP subunits and upregulation of the DR-related genes.
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影响因子：
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作者：
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