Comparison of Gene Expression Between Resistant and Susceptible Families Against VP(AHPND) and Identification of Biomarkers Used for Resistance Evaluation in Litopenaeus vannamei.
Comparison of Gene Expression Between Resistant and Susceptible Families Against VP(AHPND) and Identification of Biomarkers Used for Resistance Evaluation in Litopenaeus vannamei.
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DOI：
10.3389/fgene.2021.772442
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发表时间：
2021
影响因子：
3.7
通讯作者：
Li F
中科院分区：
文献类型：
--
作者：
Zhang Q;Yu Y;Luo Z;Xiang J;Li F
急性肝胰腺坏死病（AHPND）自爆发以来，给对虾养殖业造成了重大损失。副溶血性弧菌（VPAHPND）被认为是引起凡纳滨对虾AHPND的主要病原体之一。为了进一步了解AHPND的抗性机制，通过遗传育种获得了抗性和易感对虾家族，并采用比较转录组法分析了抗性和易感对虾家族之间的基因表达谱。对95个家系进行了VPAHPND攻毒试验，观察到这些家系的抗性存在显著差异。选择3对耐药和易感家族进行转录组测序。共筛选出至少存在2个两两比较的差异表达基因489个，其中易感家族高表达基因196个，耐药家族高表达基因293个。在这些deg中，有16个基因在所有三个两两比较中都表现出显著差异。所有27331个表达基因的基因集富集分析（GSEA）表明，抗性家族中富集了部分能量代谢过程，而易感家族中富集了信号转导和免疫系统。在检测到的家族后代中进一步确认了32个deg，其中成功验证了19个基因。本研究所鉴定的基因将有助于阐明对虾对弧菌抗性的遗传机制，并进一步为对虾抗病育种提供分子标记。
Acute hepatopancreatic necrosis disease (AHPND) has caused a heavy loss to shrimp aquaculture since its outbreak. Vibrio parahaemolyticus (VPAHPND) is regarded as one of the main pathogens that caused AHPND in the Pacific white shrimp Litopenaeus vannamei. In order to learn more about the mechanism of resistance to AHPND, the resistant and susceptible shrimp families were obtained through genetic breeding, and comparative transcriptome approach was used to analyze the gene expression patterns between resistant and susceptible families. A total of 95 families were subjected to VPAHPND challenge test, and significant variations in the resistance of these families were observed. Three pairs of resistant and susceptible families were selected for transcriptome sequencing. A total of 489 differentially expressed genes (DEGs) that presented in at least two pairwise comparisons were screened, including 196 DEGs highly expressed in the susceptible families and 293 DEGs in the resistant families. Among these DEGs, 16 genes demonstrated significant difference in all three pairwise comparisons. Gene set enrichment analysis (GSEA) of all 27,331 expressed genes indicated that some energy metabolism processes were enriched in the resistant families, while signal transduction and immune system were enriched in the susceptible families. A total of 32 DEGs were further confirmed in the offspring of the detected families, among which 19 genes were successfully verified. The identified genes in this study will be useful for clarifying the genetic mechanism of shrimp resistance against Vibrio and will further provide molecular markers for evaluating the disease resistance of shrimp in the breeding program.
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影响因子：
5.2
作者：
Kumar R;Tung TC;Ng TH;Chang CC;Chen YL;Chen YM;Lin SS;Wang HC
通讯作者：
Wang HC
文蛤抗性相关基因表达性状遗传力及其与体型的相关性
影响因子：
1.6
作者：
Jiang, Fengjuan;Yue, Xin;Wang, Hongxia
通讯作者：
Wang, Hongxia
影响因子：
10.7
作者：
Leder EH;McCairns RJ;Leinonen T;Cano JM;Viitaniemi HM;Nikinmaa M;Primmer CR;Merilä J
通讯作者：
Merilä J
影响因子：
4.7
作者：
Amparyup, Piti;Charoensapsri, Walaiporn;Tassanakajon, Anchalee
通讯作者：
Tassanakajon, Anchalee
DOI：
10.1073/pnas.1503129112
发表时间：
2015-08-25
影响因子：
11.1
作者：
Lee, Chung-Te;Chen, I-Tung;Lo, Chu-Fang
通讯作者：
Lo, Chu-Fang