Comparison of the activation of somatostatin- and neuropeptide Y-containing neuronal populations of the rat amygdala following two different anxiogenic stressors.
Comparison of the activation of somatostatin- and neuropeptide Y-containing neuronal populations of the rat amygdala following two different anxiogenic stressors.
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DOI：
10.1016/j.expneurol.2012.08.002
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发表时间：
2012-11
影响因子：
5.3
通讯作者：
Wilson MA
中科院分区：
文献类型：
--
作者：
Butler RK;White LC;Frederick-Duus D;Kaigler KF;Fadel JR;Wilson MA
老鼠暴露在捕食者的气味或升高的迷宫中，在没有事先暴露于任何刺激的情况下表现出恐惧行为。先天恐惧的表达提供了一个理想的焦虑模型，可以帮助阐明参与焦虑相关行为的大脑回路。目前的实验比较了大鼠的杏仁核中含有神经肽的神经元群的激活情况，这些大鼠暴露于升高的迷宫（EPM； 5分钟）与家庭笼子对照，或捕食者雪貂气味与丁酸或无气味（30分钟）。制备脑切片进行双标记免疫组化，并在杏仁核基底外侧（BLA）、中央（CEA）、内侧（MEA）核中计数与生长抑素（SOM）或神经肽Y （NPY）共定位的c-Fos。与对照组相比，雪貂气味和丁酸暴露显著降低了前BLA中对c-Fos具有免疫反应的som阳性神经元的百分比，而EPM暴露则显著增加了som阳性神经元的激活。在CEA中，与无气味对照组相比，雪貂气味和丁酸暴露显著降低了som阳性神经元对c-Fos的免疫反应百分比，而EPM暴露与对照组相比没有变化。在MEA中，与对照组相比，雪貂气味暴露和EPM暴露均导致SOM与c-Fos共定位增加，而NPY与c-Fos共定位发生在雪貂气味暴露后，而EPM暴露后则没有。这些结果表明，杏仁核不同的神经元群在暴露于不同的焦虑刺激后被不同地激活。这些研究进一步阐明了焦虑的基本神经回路，并可能解释捕食者和新事物引起的压力的不同行为影响。
Rats exposed to the odor of a predator or to the elevated plus maze express fear behaviors without a prior exposure to either stimulus. The expression of innate fear provides for an ideal model of anxiety which can aid in the elucidation of brain circuits involved in anxiety-related behaviors. The current experiments compared activation of neuropeptide-containing neuronal populations in the amygdala of rats exposed to either the elevated plus maze (EPM; 5 minutes) versus home cage controls, or predator ferret odor versus butyric acid, or no odor (30 minutes). Sections of the brains were prepared for dual-labeled immunohistochemistry and counts of c-Fos co-localized with somatostatin (SOM) or neuropeptide Y (NPY) were made in the basolateral (BLA), central (CEA), medial (MEA) nucleus of the amygdala. Ferret odor and butyric acid exposure significantly decreased the percentage of SOM–positive neurons also immunoreactive for c-Fos in the anterior BLA compared to controls, whereas EPM exposure yielded a significant increase in the activation of SOM-positive neurons versus home cage controls. In the CEA, ferret odor and butyric exposure significantly decreased the percentage of SOM-positive neurons also immunoreactive for c-Fos compared to no-odor controls whereas EPM exposure yielded no change versus controls. In the MEA, both ferret odor exposure and EPM exposure resulted in increased SOM co-localized with c-Fos compared to control groups whereas NPY co-localized with c-Fos occurred following ferret odor exposure, but not EPM exposure. These results indicate that phenotypically distinct neuronal populations of the amygdala are differentially activated following exposure to different anxiogenic stimuli. These studies further elucidate the fundamental neurocircuitry of anxiety and could possibly explain the differential behavioral effects of predator versus novelty-induced stress.
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DOI：
10.1111/j.1749-6632.1997.tb48289.x
发表时间：
1997-01-01
期刊：
PSYCHOBIOLOGY OF POSTTRAUMATIC STRESS DISORDER
影响因子：
--
作者：
Davis, M;Walker, DL;Lee, YL
通讯作者：
Lee, YL
DOI：
10.1098/rstb.1997.0149
发表时间：
1997-11-29
期刊：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
Davis, M;Walker, DL;Lee, Y
通讯作者：
Lee, Y
影响因子：
4.7
作者：
Chieng, Billy;Christie, MacDonald J.
通讯作者：
Christie, MacDonald J.
DOI：
10.1007/bf00495150
发表时间：
1985-01-01
期刊：
HISTOCHEMISTRY
影响因子：
--
作者：
BUCHAN, AMJ;SIKORA, LKJ;BROWN, JC
通讯作者：
BROWN, JC
影响因子：
3.6
作者：
HANDLEY, SL;MITHANI, S
通讯作者：
MITHANI, S