Jammed Micro-Flake Hydrogel for Four-Dimensional Living Cell Bioprinting.
Jammed Micro-Flake Hydrogel for Four-Dimensional Living Cell Bioprinting.
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DOI：
10.1002/adma.202109394
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发表时间：
2022-04
影响因子：
29.4
通讯作者：
Alsberg, Eben
中科院分区：
文献类型：
--
作者：
Ding, Aixiang;Jeon, Oju;Cleveland, David;Gasvoda, Kaelyn L.;Wells, Derrick;Lee, Sang Jin;Alsberg, Eben
四维（4D）生物打印有望构建具有复杂几何形状和功能的细胞负载结构（生物结构），用于组织/器官再生应用。基于水凝胶的4D生物墨水的开发，特别是那些允许活细胞打印的，具有易于制备，确定成分和控制物理性质的水凝胶生物墨水对于4D生物打印至关重要。本研究开发了一种不同于传统颗粒微凝胶，具有非均匀粒径分布的单组分堵塞微片水凝胶（MFH）体系，作为4D生物打印的新型细胞负载生物链。这种堵塞的细胞兼容MFH具有可扩展的生产和直接的组成，具有剪切减薄、剪切屈服和快速自愈的特性。因此，它可以顺利地打印成稳定的3D生物结构，当光引发剂和紫外线吸收剂结合时，可以进一步交联以形成交联密度的梯度。经过形状变形后，获得了各种结构明确、细胞活力高的复杂生物结构。基于该系统，4D软骨样组织形成被证明是一个概念验证。这种多用途的新型4D生物链接系统的建立可能在组织工程中开辟许多应用。开发了单组分堵塞微片水凝胶（MFHs）作为4D生物打印的细胞负载生物墨水。不需要额外填料的细胞相容性MFH生物墨水在流变学上有利于通过平滑的直接墨水书写（DIW）进行生物打印。在3D打印生物结构中实现了可控的交联梯度，从而实现了预定义的形状转换。最终，4D组织工程在4D软骨样组织再生研究的概念验证中得到证明。
Four-dimensional (4D) bioprinting is promising to build cell-laden constructs (bioconstructs) with complex geometries and functions for tissue/organ regeneration applications. The development of hydrogel-based 4D bioinks, especially those allowing living cell printing, with easy preparation, defined composition, and controlled physical properties is critically important for 4D bioprinting. Here, a single-component jammed micro-flake hydrogel (MFH) system with heterogeneous size distribution, which differs from the conventional granular microgel, has been developed as a new cell-laden bioink for 4D bioprinting. This jammed cytocompatible MFH features scalable production and straightforward composition with shear-thinning, shear-yielding, and rapid self-healing properties. As such, it can be smoothly printed into stable 3D bioconstructs, which can be further crosslinked to form a gradient in crosslinking density when a photoinitiator and a UV absorber are incorporated. After being subject to shape morphing, a variety of complex bioconstructs with well-defined configurations and high cell viability were obtained. Based on this system, 4D cartilage-like tissue formation was demonstrated as a proof-of-concept. The establishment of this versatile new 4D bioink system may open up a number of applications in tissue engineering. Single-component jammed micro-flake hydrogels (MFHs) were developed as cell-laden bioinks for 4D bioprinting. Cytocompatible MFH bioinks without the need of additional fillers are rheologically favorable for bioprinting via smooth direct ink writing (DIW). A controllable crosslinking gradient in the 3D printed bioconstructs was achieved, enabling predefined shape transformations. Ultimately, 4D tissue engineering was demonstrated in a proof-of-concept 4D cartilage-like tissue regeneration study.
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影响因子：
18.9
作者：
Elkhoury K;Morsink M;Sanchez-Gonzalez L;Kahn C;Tamayol A;Arab-Tehrany E
通讯作者：
Arab-Tehrany E
影响因子：
4.7
作者：
Ashammakhi N;Ahadian S;Zengjie F;Suthiwanich K;Lorestani F;Orive G;Ostrovidov S;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
29.4
作者：
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通讯作者：
Ionov, Leonid
影响因子：
41.2
作者：
Gladman, A. Sydney;Matsumoto, Elisabetta A.;Lewis, Jennifer A.
通讯作者：
Lewis, Jennifer A.
影响因子：
18.9
作者：
Ding A;Lee SJ;Ayyagari S;Tang R;Huynh CT;Alsberg E
通讯作者：
Alsberg E