Patterns of expression in the matrix proteins responsible for nucleation and growth of aragonite crystals in flat pearls of Pinctada fucata.
Patterns of expression in the matrix proteins responsible for nucleation and growth of aragonite crystals in flat pearls of Pinctada fucata.
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珠母贝扁平珍珠文石晶体成核和生长基质蛋白的表达模式
DOI：
10.1371/journal.pone.0066564
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhang R
中科院分区：
文献类型：
--
作者：
Xiang L;Su J;Zheng G;Liang J;Zhang G;Wang H;Xie L;Zhang R
珍珠层的初始生长对理解珍珠文石的形成至关重要。在内壳膜存在的情况下，采用扁平珍珠法评价基质蛋白在珠层生物矿化初始阶段的作用。我们检查了沉积在底物上的晶体和基质蛋白在面向底物的地幔中的表达模式。在本研究中，文石晶体在内壳膜体系中5天后在表面成核。在无膜体系中，方解石晶体在第5天成核，方解石被新的有机膜覆盖，文石在第10天成核。这意味着内壳膜体系中珍珠层的出现时间比无膜体系早5天，这与前20天基质蛋白的最高水平一致。此外，基质蛋白（Nacrein, MSI60, N19， N16和Pif80）在膜内系统中控制珍珠生长的序列形态具有相似的表达模式，而这些蛋白在无膜系统中也具有相似的表达模式。这些结果表明，基质蛋白在体内通过内壳膜调控文石的成核和生长。
The initial growth of the nacreous layer is crucial for comprehending the formation of nacreous aragonite. A flat pearl method in the presence of the inner-shell film was conducted to evaluate the role of matrix proteins in the initial stages of nacre biomineralization in vivo. We examined the crystals deposited on a substrate and the expression patterns of the matrix proteins in the mantle facing the substrate. In this study, the aragonite crystals nucleated on the surface at 5 days in the inner-shell film system. In the film-free system, the calcite crystals nucleated at 5 days, a new organic film covered the calcite, and the aragonite nucleated at 10 days. This meant that the nacre lamellae appeared in the inner-shell film system 5 days earlier than that in the film-free system, timing that was consistent with the maximum level of matrix proteins during the first 20 days. In addition, matrix proteins (Nacrein, MSI60, N19, N16 and Pif80) had similar expression patterns in controlling the sequential morphologies of the nacre growth in the inner-film system, while these proteins in the film-free system also had similar patterns of expression. These results suggest that matrix proteins regulate aragonite nucleation and growth with the inner-shell film in vivo.
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影响因子：
3.7
作者：
Fang D;Xu G;Hu Y;Pan C;Xie L;Zhang R
通讯作者：
Zhang R
影响因子：
3
作者：
Inoue, Nariaki;Ishibashi, Ryo;Komaru, Akira
通讯作者：
Komaru, Akira
影响因子：
3.5
作者：
Samata, T;Hayashi, N;Akera, S
通讯作者：
Akera, S
基质蛋白在珍珠形成过程中控制珍珠层沉积的作用
DOI：
10.1098/rspb.2011.1661
发表时间：
2012-03-07
影响因子：
4.7
作者：
Liu, Xiaojun;Li, Jiale;Zhang, Rongqing
通讯作者：
Zhang, Rongqing
影响因子：
3.2
作者：
Gotliv, BA;Addadi, L;Weiner, S
通讯作者：
Weiner, S