A Printed Multicomponent Paper Sensor for Bacterial Detection.

A Printed Multicomponent Paper Sensor for Bacterial Detection.
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DOI:
10.1038/s41598-017-12549-3
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发表时间:
2017-09-26
期刊:
影响因子:
4.6
通讯作者:
Brennan JD
Brennan JD
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Ali MM;Brown CL;Jahanshahi-Anbuhi S;Kannan B;Li Y;Filipe CDM;Brennan JD

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We present a simple all-in-one paper-based sensor for E. coli detection using a composite ink made of a fluorogenic DNAzyme probe for bacterial recognition and signal generation, lysozyme that lyses whole bacterial cells, and pullulan/trehalose sugars that stabilize printed bioactive molecules. The paper sensor is capable of producing a fluorescence signal as a readout within 5 minutes upon contacting E. coli, can achieve a limit of detection of 100 cells/mL, in a variety of sample matrixes, without sample enrichment, and remains stable for at least 6 months when stored at ambient temperature. Therefore, this simple paper sensor provides rapid bacterial testing on site, and can be shipped and stored under ambient conditions to benefit users living in resource-limited regions.
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