Injectable and porous PLGA microspheres that form highly porous scaffolds at body temperature.
Injectable and porous PLGA microspheres that form highly porous scaffolds at body temperature.
复制标题
DOI：
10.1016/j.actbio.2014.08.015
复制
发表时间：
2014-12
影响因子：
9.7
通讯作者：
Rahman, Cheryl V.
中科院分区：
文献类型：
--
作者：
Qutachi, Omar;Vetsch, Jolanda R.;Gill, Daniel;Cox, Helen;Scurr, David J.;Hofmann, Sandra;Mueller, Ralph;Quirk, Robin A.;ShakeSheff, Kevin M.;Rahman, Cheryl V.
可注射支架由于其微创的输送方式，在再生医学领域引起了人们的兴趣。对于组织修复应用，这种支架必须具有支持新组织形成的机械性能、孔隙率和孔径。在本研究中，制备了平均粒径为84±0.24μm的聚乳酸-乙醇酸共聚微球作为可注射细胞载体。观察到，用乙醇氢氧化钠处理2分钟可以增加表面孔隙率，而不会导致微球结构解体。这种表面处理还使微球在37℃下融合在一起形成支架结构。支架在37℃下放置24小时后的平均抗压强度为0.9±0.1 Mpa，平均杨氏模数为9.4±11.2 Mpa。支架的平均孔隙率为81.6%，平均孔径为54±3.38μm。本研究介绍了一种制备多孔聚乳酸微球的方法，该微球在体温下形成固体多孔支架，创建了一个能够支持NIH-3T3细胞体外附着和增殖的注射系统。
Injectable scaffolds are of interest in the field of regenerative medicine because of their minimally invasive mode of delivery. For tissue repair applications, it is essential that such scaffolds have the mechanical properties, porosity and pore diameter to support the formation of new tissue. In the current study, porous poly(dl-lactic acid-co-glycolic acid) (PLGA) microspheres were fabricated with an average size of 84 ± 24 μm for use as injectable cell carriers. Treatment with ethanolic sodium hydroxide for 2 min was observed to increase surface porosity without causing the microsphere structure to disintegrate. This surface treatment also enabled the microspheres to fuse together at 37 °C to form scaffold structures. The average compressive strength of the scaffolds after 24 h at 37 °C was 0.9 ± 0.1 MPa, and the average Young’s modulus was 9.4 ± 1.2 MPa. Scaffold porosity levels were 81.6% on average, with a mean pore diameter of 54 ± 38 μm. This study demonstrates a method for fabricating porous PLGA microspheres that form solid porous scaffolds at body temperature, creating an injectable system capable of supporting NIH-3T3 cell attachment and proliferation in vitro.
登录
查看更多内容
影响因子：
14
作者：
van Lenthe, G. Harry;Hagenmueller, Henri;Mueller, Ralph
通讯作者：
Mueller, Ralph
DOI：
10.1002/jbm.b.31561
发表时间：
2010-04-01
影响因子：
3.4
作者：
Wang, Yingjun;Shi, Xuetao;Wang, Dong-An
通讯作者：
Wang, Dong-An
影响因子：
6.2
作者：
Hildebrand, T;Laib, A;Rüegsegger, P
通讯作者：
Rüegsegger, P
影响因子：
3.1
作者：
Murphy, Ciara M.;O'Brien, Fergal J.;Schindeler, Aaron
通讯作者：
Schindeler, Aaron
影响因子：
14
作者：
Jiang, Tao;Abdel-Fattah, Wafa I.;Laurencin, Cato T.
通讯作者：
Laurencin, Cato T.