Relative Frequency of Blastocystis Subtypes 1, 2, and 3 in Urban and Periurban Human Populations of Arequipa, Peru.
Relative Frequency of Blastocystis Subtypes 1, 2, and 3 in Urban and Periurban Human Populations of Arequipa, Peru.
复制标题
秘鲁阿雷基帕城市和城郊人群中芽囊原虫亚型1、2和3的相对频率。
DOI：
10.3390/tropicalmed5040178
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发表时间：
2020-11-27
影响因子：
2.9
通讯作者：
Ballón-Echegaray J
中科院分区：
文献类型：
--
作者：
Ascuña-Durand K;Salazar-Sánchez RS;Castillo-Neyra R;Ballón-Echegaray J
芽囊原虫是在人类肠道中发现的最常见的原虫之一，具有遗传多样性，广泛分布于世界各地。非特异性和不一致的症状与这种原虫有关；因此，它的临床重要性仍然存在争议。我们的目的是根据SSU rDNA序列的保守区，估计南美洲报道的主要亚型1、2和3的相对频率，并确定与它们相关的因素。对116份芽囊原虫阳性粪便标本进行常规聚合酶链式反应(PCR)扩增。我们确定了亚型1(10.3%)、亚型2(7.8%)、亚型3(25.0%)和混合亚型感染(8.7%)。然而，我们无法在48.3%的样本中识别出任何芽囊原虫亚型；因此，很可能该地区存在其他亚型。没有发现任何胃肠道症状与单一或混合的芽囊原虫亚型有关。我们发现芽囊原虫亚型2与肠易激综合征有统计学意义的相关性(OR=17.8，95%CI=1.5-408.4，p=0.039)；然而，患有肠易激综合征的样本数量很少(n=4)。芽囊原虫亚型与所研究的任何流行病学变量之间没有关联。在农村人口中，我们只确定了亚型1，而在城市和城市周边人口中，我们确定了亚型1、2和3。
Blastocystis is one of the most common protozoa found in the human gut and are genetically diverse and widely distributed around the world. Nonspecific and inconsistent symptoms have been associated with this protozoon; thus, its clinical importance remains controversial. Our aim was to estimate the relative frequency of Blastocystis subtypes 1, 2, and 3, which are the predominant subtypes reported in South America, based on conserved regions of SSU rDNA sequences and determine the factors associated with them. A total of 116 Blastocystis-positive stool samples were processed using conventional PCR with Blastocystis-specific primers. We identified subtype 1 (10.3%), subtype 2 (7.8%), subtype 3 (25.0%), and mixed subtype infections (8.7%). However, we could not identify any Blastocystis subtypes in 48.3% of the samples; therefore, it is likely that other subtypes were present in the area. No association was found between any gastrointestinal symptom and single or mixed Blastocystis subtypes. We found a statistically significant association between Blastocystis subtype 2 and irritable bowel syndrome (OR = 17.8, 95% CI = 1.5–408.4, p = 0.039); however, the number of samples with IBS was small (n = 4). There was no association between the Blastocystis subtypes and any epidemiological variable studied. In rural populations, we only identified subtype 1, while in urban and periurban populations, we identified subtypes 1, 2, and 3.
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影响因子：
3.7
作者：
Barbosa CV;Barreto MM;Andrade RJ;Sodré F;d'Avila-Levy CM;Peralta JM;Igreja RP;de Macedo HW;Santos HLC
通讯作者：
Santos HLC
影响因子：
1.9
作者：
Dogan, Nihal;Aydin, Merve;Dogruman-Al, Funda
通讯作者：
Dogruman-Al, Funda
影响因子：
3.7
作者：
David Ramirez, Juan;Florez, Carolina;Cesar Giraldo, Julio
通讯作者：
Cesar Giraldo, Julio
影响因子：
2.7
作者：
Alfellani, Mohammed A.;Stensvold, C. Rune;Clark, C. Graham
通讯作者：
Clark, C. Graham
影响因子：
4.7
作者：
Cifre, Susana;Gozalbo, Monica;Trelis, Maria
通讯作者：
Trelis, Maria