Effects of Momordica charantia (Bitter Melon) on Ischemic Diabetic Myocardium.
Effects of Momordica charantia (Bitter Melon) on Ischemic Diabetic Myocardium.
复制标题
DOI：
10.3390/molecules22030488
复制
发表时间：
2017-03-20
期刊：
影响因子：
--
通讯作者：
Lekli I
中科院分区：
文献类型：
--
作者：
Czompa A;Gyongyosi A;Szoke K;Bak I;Csepanyi E;Haines DD;Tosaki A;Lekli I
目的：在此使用大鼠模型来检验苦瓜（苦瓜（BM））提取物有利地改变心血管组织中的过程并且与2型糖尿病（T2 DM）和相关心血管疾病的病理生理学系统相关的假设。研究方法：雄性瘦和Zucker肥胖（ZO）大鼠用400 mg/kg体重的苦瓜（BM）提取物（悬浮在粘蛋白-水载体中）或载体（对照）管饲治疗6周。根据品系（Lean或ZO）和处理（对照或BM），将动物分为4个处理组，每组10只动物。在六周的治疗期后，从所选动物收集外周血，然后处死、开胸并安装分离的工作心脏装置。结果如下：Lean和ZO大鼠的体重均不受给药影响，同样，外周血空腹血糖水平未显示出显著的给药相关效应。然而，当将瘦型、BM处理的动物与溶剂处理的瘦型对照大鼠进行比较时，在缺血后心脏功能中注意到一些BM处理相关的改善。瘦，但不ZO，大鼠的治疗显着降低了缺血30分钟，然后2小时的工作模式再灌注的离体心脏的梗死区的大小。免疫组织化学证明的半胱天冬酶-3表达的离体心脏组织进行30分钟的缺血，然后2小时的再灌注，揭示了BM治疗和减少表达的这种酶从瘦和ZO动物的心脏之间的显着相关性。caspase-3表达的等级和顺序从最高到最低如下：接受媒介物的ZO大鼠>接受BM提取物的ZO大鼠>接受媒介物处理的瘦大鼠>施用BM提取物的瘦大鼠。心脏相关分析的外周血含量分析结果：与临床应用特别相关的是ZO和ZO BM治疗的血液中总胆固醇（高密度脂蛋白HDL-c +低密度脂蛋白LDL-c）相对于瘦大鼠显著升高，推测HDL-c/LDL-c比值增加-与动脉粥样硬化疾病风险降低相关的结果。结论：BM提取物未能在建议用作独立治疗的水平上对T2 DM和心血管相关结局产生积极影响。然而，BM在增强心脏功能、抑制缺血/再灌注后梗死面积范围和调节血清胆固醇能力方面的令人鼓舞的作用可能使其可用作T2 DM和相关心血管疾病管理的辅助治疗。
Objective: A rat model is here used to test a hypothesis that Momordica charantia (Bitter melon (BM)) extract favorably alters processes in cardiovascular tissue and is systemically relevant to the pathophysiology of type 2 diabetes (T2DM) and related cardiovascular disease. Methods: Male Lean and Zucker Obese (ZO) rats were gavage-treated for six weeks with 400 mg/kg body weight bitter melon (BM) extract suspended in mucin–water vehicle, or with vehicle (Control). Animals were segregated into four treatment groups, 10 animals in each group, according to strain (Lean or ZO) and treatment (Control or BM). Following six-week treatment periods, peripheral blood was collected from selected animals, followed by sacrifice, thoracotomy and mounting of isolated working heart setup. Results: Body mass of both Lean and ZO rats was unaffected by treatment, likewise, peripheral blood fasting glucose levels showed no significant treatment-related effects. However, some BM treatment-related improvement was noted in postischemic cardiac functions when Lean, BM-treated animals were compared to vehicle treated Lean control rats. Treatment of Lean, but not ZO, rats significantly reduced the magnitude of infarcted zone in isolated hearts subjected to 30 min of ischemia followed by 2 h of working mode reperfusion. Immunohistochemical demonstration of caspase-3 expression by isolated heart tissues subjected to 30 min of ischemia followed by 2 h of reperfusion, revealed significant correlation between BM treatment and reduced expression of this enzyme in hearts obtained from both Lean and ZO animals. The hierarchy and order of caspase-3 expression from highest to lowest was as follows: ZO rats receiving vehicle > ZO rats receiving BM extract > Lean rats treated receiving vehicle > Lean rats administered BM extract. Outcomes of analyses of peripheral blood content of cardiac-related analytics: with particular relevance to clinical application was a significant elevation in blood of ZO and ZO BM-treated, versus Lean rats of total cholesterol (high density lipoprotein HDL-c + low density lipoprotein LDL-c), with an inferred increase in HDL-c/LDL-c ratio—an outcome associated with decreased risk of atherosclerotic disease. Conclusions: BM extract failed to positively affect T2DM- and cardiovascular-related outcomes at a level suggesting use as a standalone treatment. Nevertheless, the encouraging effects of BM in enhancement of cardiac function, suppression of post-ischemic/reperfused infarct size extent and capacity to modulate serum cholesterol, will likely make it useful as an adjuvant therapy for the management of T2DM and related cardiovascular diseases.
登录
查看更多内容
DOI：
10.1002/food.19950390403
发表时间：
1995-01-01
期刊：
NAHRUNG-FOOD
影响因子：
--
作者：
PLATEL, K;SRINIVASAN, K
通讯作者：
SRINIVASAN, K
DOI：
10.1152/ajpheart.01048.2007
发表时间：
2008-02-01
影响因子：
4.8
作者：
Lekli, Istvan;Szabo, Gergo;Tosaki, Arpad
通讯作者：
Tosaki, Arpad
DOI：
10.1016/j.bbadis.2014.04.029
发表时间：
2015-02
影响因子：
6.2
作者：
Ren, Sidney Y.;Xu, Xihui
通讯作者：
Xu, Xihui
影响因子：
5.3
作者：
Alam MA;Uddin R;Subhan N;Rahman MM;Jain P;Reza HM
通讯作者：
Reza HM
DOI：
10.1016/s2222-1808(13)60052-3
发表时间：
2013-01-01
期刊：
Asian Pacific Journal of Tropical Disease
影响因子：
--
作者：
Baby Joseph, Baby Joseph;Jini, D.;Joseph, B.
通讯作者：
Joseph, B.