Environmental Stability of Plasmonic Biosensors Based on Natural versus Artificial Antibody.
Environmental Stability of Plasmonic Biosensors Based on Natural versus Artificial Antibody.
复制标题
基于自然抗体的等离激子生物传感器的环境稳定性。
DOI：
10.1021/acs.analchem.7b05470
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发表时间：
2018-07-03
影响因子：
7.4
通讯作者：
Singamaneni S
中科院分区：
文献类型：
--
作者：
Luan J;Xu T;Cashin J;Morrissey JJ;Kharasch ED;Singamaneni S
基于局域表面等离子体共振（LSPR）的折射率灵敏度的等离子体生物传感器被认为是非常有前途的芯片上和即时生物诊断。然而，大多数当前的等离子体生物传感器采用天然抗体作为生物识别元件，其在暴露于环境应激源（例如，温度和湿度）。等离子体生物传感器依赖于分子印迹作为识别元件（人工抗体）被假设为是一个有吸引力的替代应用在资源有限的设置，由于其优异的热，化学和环境稳定性。在这项工作中，我们提供了一个全面的等离子体生物传感器的稳定性的比较基于天然和人工抗体。尽管基于天然抗体的等离子体生物传感器表现出上级灵敏度，但发现它们的稳定性（时间、热和化学）大大劣于基于人工抗体的那些。我们的研究结果令人信服地证明，这些新型的基于人工抗体的等离子体生物传感器对于护理点和资源有限的条件是非常有吸引力的，在这些条件下，对运输、储存和处理条件的严格控制是不可能的。
Plasmonic biosensors based on the refractive index sensitivity of localized surface plasmon resonance (LSPR) are considered to be highly promising for on-chip and point-of-care biodiagnostics. However, most of the current plasmonic biosensors employ natural antibodies as biorecognition elements, which can easily lose their biorecognition ability upon exposure to environmental stressors (e.g., temperature and humidity). Plasmonic biosensors relying on molecular imprints as recognition elements (artificial antibodies) are hypothesized to be an attractive alternative for applications in resource-limited settings due to their excellent thermal, chemical, and environmental stability. In this work, we provide a comprehensive comparison of the stability of plasmonic biosensors based on natural and artificial antibodies. Although the natural antibody-based plasmonic biosensors exhibit superior sensitivity, their stability (temporal, thermal, and chemical) was found to be vastly inferior to those based on artificial antibodies. Our results convincingly demonstrate that these novel classes of artificial antibody-based plasmonic biosensors are highly attractive for point-of-care and resource-limited conditions where tight control over transport, storage, and handling conditions is not possible.
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影响因子：
14
作者：
Shiomi, T;Matsui, M;Sakaguchi, K
通讯作者：
Sakaguchi, K
影响因子：
4.6
作者：
Tadepalli S;Kuang Z;Jiang Q;Liu KK;Fisher MA;Morrissey JJ;Kharasch ED;Slocik JM;Naik RR;Singamaneni S
通讯作者：
Singamaneni S
影响因子：
7
作者：
Tian, Limei;Liu, Keng-Ku;Singamaneni, Srikanth
通讯作者：
Singamaneni, Srikanth
单分子力光谱比较自然与人工抗体抗原相互作用。
DOI：
10.1002/smll.201604255
发表时间：
2017-05
期刊：
Small (Weinheim an der Bergstrasse, Germany)
影响因子：
--
作者：
Wang C;Hu R;Morrissey JJ;Kharasch ED;Singamaneni S
通讯作者：
Singamaneni S
影响因子：
19
作者：
Abbas, Abdennour;Tian, Limei;Morrissey, Jeremiah J.;Kharasch, Evan D.;Singamaneni, Srikanth
通讯作者：
Singamaneni, Srikanth