The hemolymph of Biomphalaria snail vectors of schistosomiasis supports a diverse microbiome.
The hemolymph of Biomphalaria snail vectors of schistosomiasis supports a diverse microbiome.
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DOI：
10.1111/1462-2920.15303
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发表时间：
2020-12
影响因子：
5.1
通讯作者：
Le Clec'h W
中科院分区：
文献类型：
--
作者：
Chevalier FD;Diaz R;McDew-White M;Anderson TJC;Le Clec'h W
微生物组-在生物体上或体内发现的微生物群落-越来越多地被认识到塑造其宿主生物学的许多方面，并且是健康和疾病的关键决定因素。微生物组调节昆虫病媒（蚊子、采采蝇、白蛉）传播寄生虫和疾病的能力。我们调查的多样性和丰富的微生物内的血淋巴（即血液）的双脐螺，中间宿主曼氏血吸虫，使用Illumina MiSeq测序的细菌16 S V4 rDNA。我们从B的6个不同的实验室种群的5个螺的血淋巴中取样。glabrata和一个B种群。亚历山德拉。我们观察到每只蜗牛有279.84 ± 0.79个扩增子序列变异（ASV）。在个体蜗牛、蜗牛种群和物种水平上，微生物组组成存在显着差异。螺体内微生物群落以变形菌门和拟杆菌门为主，螺池水体微生物群落以放线菌为主。我们使用qPCR研究了绝对细菌载量：血淋巴样品含有2，784 ± 339个细菌/μL。我们推测，微生物组可能代表了蜗牛-寄生虫相互作用中一个关键但未探索的中介，因为血淋巴在其发育的每个阶段都与寄生虫非常密切地接触。
The microbiome – the microorganism community that is found on or within an organism’s body – is increasingly recognized to shape many aspects of its host biology and is a key determinant of health and disease. Microbiomes modulate the capacity of insect disease vectors (mosquitoes, tsetse flies, sandflies) to transmit parasites and disease. We investigate the diversity and abundance of microorganisms within the hemolymph (i.e. blood) of Biomphalaria snails, the intermediate host for Schistosoma mansoni, using Illumina MiSeq sequencing of the bacterial 16S V4 rDNA. We sampled hemolymph from 5 snails from 6 different laboratory populations of B. glabrata and one population of B. alexandrina. We observed 279.84 ± 0.79 amplicon sequence variants (ASVs) per snail. There were significant differences in microbiome composition at the level of individual snails, snail populations and species. Snail microbiomes were dominated by Proteobacteria and Bacteroidetes while water microbiomes from snail tank were dominated by Actinobacteria. We investigated the absolute bacterial load using qPCR: hemolymph samples contained 2,784 ± 339 bacteria per μL. We speculate that the microbiome may represent a critical, but unexplored intermediary in the snail-schistosome interaction as hemolymph is in very close contact to the parasite at each step of its development.
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影响因子：
3.7
作者：
Braquart-Varnier C;Lachat M;Herbinière J;Johnson M;Caubet Y;Bouchon D;Sicard M
通讯作者：
Sicard M
影响因子：
1.4
作者：
Apprill, Amy;McNally, Sean;Weber, Laura
通讯作者：
Weber, Laura
影响因子：
6.4
作者：
Dheilly NM;Bolnick D;Bordenstein S;Brindley PJ;Figuères C;Holmes EC;Martínez Martínez J;Phillips AJ;Poulin R;Rosario K
通讯作者：
Rosario K
影响因子：
3.6
作者：
Antunes, Filipa;Hinzmann, Mariana;da Costa, Paulo Martins
通讯作者：
da Costa, Paulo Martins
影响因子：
3.1
作者：
Allan, Euan R. O.;Tennessen, Jacob A.;Blouin, Michael S.
通讯作者：
Blouin, Michael S.