Up-regulation of ski-interacting protein in rat brain cortex after traumatic brain injury
Up-regulation of ski-interacting protein in rat brain cortex after traumatic brain injury
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脑外伤后大鼠大脑皮层滑雪相互作用蛋白的上调
DOI：
10.1007/s10735-012-9444-9
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发表时间：
2012-09
影响因子：
3.2
通讯作者：
Shi, Wei
中科院分区：
文献类型：
--
作者：
Ni, Lanchun;Ke, Kaifu;Cao, MaoHong;Shi, Wei
SKIP(Ski-Interaction Protein)是核调控复合体的一部分，与参与预起始、剪接和多聚腺苷化的因子相互作用，增强重要转录因子的活性，参与越来越多的信号级联反应。然而，它在中枢神经系统中的分布和功能仍然知之甚少。在本研究中，Western印迹分析、RT-PCR和免疫组织化学显示，同侧创伤周围皮质与假手术组相比，SKIP的表达显著上调。免疫荧光标记显示Skip蛋白定位于神经元，而不是星形胶质细胞和少突胶质细胞；Skip蛋白与活化的caspase-3和增殖细胞核抗原共同定位于同侧皮质。此外，活跃的caspase-3和增殖细胞核抗原的表达模式与SKIP的表达模式是平行的。根据我们的数据，我们推测Skip可能在脑创伤后神经细胞的凋亡中发挥重要作用，并可能为进一步研究其在创伤性脑损伤细胞周期重入中的作用提供基础。
SKIP (Ski-interacting protein), is part of nuclear regulatory complexes and interacts with factors involved in preinitiation, splicing and polyadenylation, potentiates the activity of important transcription factors, involved in an increasing number of signaling cascades. However, its distribution and function in the central nervous system remains poorly understood. In this study, western blot analysis, RT-PCR and immunohistochemistry showed a significant up-regulation of SKIP in ipsilateral peritrauma cortex compared with the sham group. Immunofluorescent labeling indicated that SKIP was localized striking in the neurons, but not astrocytes and oligodendrocytes; co-localization of SKIP and active caspase-3 and PCNA in the ipsilateral cortex. In addition, the expression patterns of active caspase-3 and PCNA were parallel with that of SKIP. Based on our data, we speculated that SKIP might play an important role in neuronal apoptosis following TBI; and might provide a basis for the further study on its role in cell cycle re-entry in traumatic brain injury.
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影响因子：
4.2
作者：
Kernie, SG;Erwin, TM;Parada, LF
通讯作者：
Parada, LF
影响因子：
3.2
作者：
Shengyang Jiang;Xiaohong Wu;Yaohua Yan;Jian Xu;Bai Shao;Xun Zhuang;Ying-ying Han;X. Gu
通讯作者：
Shengyang Jiang;Xiaohong Wu;Yaohua Yan;Jian Xu;Bai Shao;Xun Zhuang;Ying-ying Han;X. Gu
影响因子：
3.2
作者：
Zhiqiang Liu;Dongling Wang;Bai Shao;Xiaohong Wu;Jian Xu;Q. Lu;Yidan Wang;Chunmiao Li;A. She
通讯作者：
Zhiqiang Liu;Dongling Wang;Bai Shao;Xiaohong Wu;Jian Xu;Q. Lu;Yidan Wang;Chunmiao Li;A. She
DOI：
10.1523/jneurosci.13-07-02930.1993
发表时间：
1993-07
期刊：
--
影响因子：
--
作者：
HJ Okano;D. Pfaff;R. Gibbs
通讯作者：
HJ Okano;D. Pfaff;R. Gibbs
脑外伤后大鼠大脑皮层BAG-1表达增加
DOI：
10.1007/s10735-012-9408-0
发表时间：
2012-04
期刊：
J Mol Histol.
影响因子：
--
作者：
Xu T, Wang X,Gong P, Ke K, Gu X.
通讯作者：
Xu T, Wang X,Gong P, Ke K, Gu X.