The Complete Genome of a New Betabaculovirus from Clostera anastomosis
The Complete Genome of a New Betabaculovirus from Clostera anastomosis
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新月形藻吻合的新型β杆状病毒的完整基因组
DOI：
10.1371/journal.pone.0132792
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发表时间：
2015-07
期刊：
影响因子：
3.7
通讯作者：
Hu Zhihong
中科院分区：
文献类型：
--
作者：
Yin Feifei;Zhu Zheng;Liu Xiaoping;Hou Dianhai;Wang Jun;Zhang Lei;Wang Manli;Kou Zheng;Wang Hualin;Deng Fei;Hu Zhihong
分月扇舟蛾（Clostera anastomosis）是一种食叶性森林害虫。分月扇舟蛾颗粒病毒B（ClasGV B）属于杆状病毒科倍杆状病毒属，已被用于害虫的生物防治。在这里，我们报道了ClasGV-B的全基因组序列，并将其与其他先前测序的杆状病毒进行了比较。环状双链DNA基因组长度为107，439 bp，G+C含量为37.8%，包含123个开放阅读框（ORF），占基因组的93%。ClasGV-B包含37个杆状病毒核心基因、25个鳞翅目杆状病毒特异基因、19个β杆状病毒特异基因、39个与杆状病毒同源的其他基因和3个ClasGV-B特有的ORF。ClasGV-B基因组中不存在hr重复序列，但有2个非hr重复序列，基于73个杆状病毒基因组的37个核心基因构建的系统发育树将ClasGV-B归入β-杆状病毒的进化支B，与Erinnyis ello GV（ErelGV）的亲缘关系最近。ClasGV-B与ErelGV的基因排列也具有最强的共线性，但与分月扇舟蛾GV（ClanGV）、分月扇舟蛾GV-A（ClasGV-A，以前也被称为CaLGV）和Aporema Epinotia GV（EpapGV）不同，具有20 kb的倒位。ClasGV-B基因组含有三个拷贝的多角体包膜蛋白基因（polyhedron envelope protein gene，PEP），系统发育树将病毒PEP分为PEP-1、PEP-2和PEP/P10三个主要分支。ClasGV-B还含有P10的三个同源物，它们都具有N-末端卷曲螺旋结构域和C-末端基本序列。ClasGV-B编码三种成纤维细胞生长因子（FGF）同源物，其在所有测序的β杆状病毒中是保守的。系统发育分析将这三个FGF分为不同的组，并建议FGF在β-杆状病毒扩增的早期阶段进化。ClasGV-B在序列和基因排列上与已报道的Notodontidae中的ClasGV-A和ClanGV不同，表明该病毒是一种新的Notodontid β杆状病毒。
Clostera anastomosis (Lepidoptera: Notodontidae) is a defoliating forest insect pest. Clostera anastomosis granulovirus-B (ClasGV-B) belonging to the genus Betabaculovirus of family Baculoviridae has been used for biological control of the pest. Here we reported the full genome sequence of ClasGV-B and compared it to other previously sequenced baculoviruses. The circular double-stranded DNA genome is 107,439 bp in length, with a G+C content of 37.8% and contains 123 open reading frames (ORFs) representing 93% of the genome. ClasGV-B contains 37 baculovirus core genes, 25 lepidopteran baculovirus specific genes, 19 betabaculovirus specific genes, 39 other genes with homologues to baculoviruses and 3 ORFs unique to ClasGV-B. Hrs appear to be absent from the ClasGV-B genome, however, two non-hr repeats were found. Phylogenetic tree based on 37 core genes from 73 baculovirus genomes placed ClasGV-B in the clade b of betabaculoviruses and was most closely related to Erinnyis ello GV (ErelGV). The gene arrangement of ClasGV-B also shared the strongest collinearity with ErelGV but differed from Clostera anachoreta GV (ClanGV), Clostera anastomosis GV-A (ClasGV-A, previously also called CaLGV) and Epinotia aporema GV (EpapGV) with a 20 kb inversion. ClasGV-B genome contains three copies of polyhedron envelope protein gene (pep) and phylogenetic tree divides the PEPs of betabaculoviruses into three major clades: PEP-1, PEP-2 and PEP/P10. ClasGV-B also contains three homologues of P10 which all harbor an N-terminal coiled-coil domain and a C-terminal basic sequence. ClasGV-B encodes three fibroblast growth factor (FGF) homologues which are conserved in all sequenced betabaculoviruses. Phylogenetic analysis placed these three FGFs into different groups and suggested that the FGFs were evolved at the early stage of the betabaculovirus expansion. ClasGV-B is different from previously reported ClasGV-A and ClanGV isolated from Notodontidae in sequence and gene arrangement, indicating the virus is a new notodontid betabaculovirus.
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影响因子：
5.5
作者：
Xiulian Sun;G. Zhang;Z. Zhang;Zhìhóng Hú;J. Vlak;B. Arif
通讯作者：
Xiulian Sun;G. Zhang;Z. Zhang;Zhìhóng Hú;J. Vlak;B. Arif
影响因子：
3
作者：
Li Haixia;W. Zhiying;Guo Shuping;Xie Shuping
通讯作者：
Li Haixia;W. Zhiying;Guo Shuping;Xie Shuping
DOI：
--
发表时间：
2002
期刊：
--
影响因子：
--
作者：
J. Klein;M. Fasshauer;H. Klein;M. Benito;C. Kahn
通讯作者：
J. Klein;M. Fasshauer;H. Klein;M. Benito;C. Kahn
影响因子：
3.4
作者：
M. V. van Oers;J. Vlak
通讯作者：
M. V. van Oers;J. Vlak
DOI：
10.1007/978-1-4899-1834-5_11
发表时间：
1997
期刊：
--
影响因子：
--
作者：
D. O'reilly
通讯作者：
D. O'reilly