Imaging the material properties of bone specimens using reflection-based infrared microspectroscopy.
Imaging the material properties of bone specimens using reflection-based infrared microspectroscopy.
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DOI：
10.1021/ac203375d
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发表时间：
2012-04-17
影响因子：
7.4
通讯作者：
Miller, Lisa M.
中科院分区：
文献类型：
--
作者：
Acerbo, Alvin S.;Carr, G. Lawrence;Judex, Stefan;Miller, Lisa M.
傅里叶变换红外光谱(FTIRM)是一种广泛使用的方法，用于绘制骨骼和其他矿化组织的材料属性，包括矿化、结晶度、碳酸盐取代和胶原交联。这项技术传统上是以变速箱为基础的几何结构，这需要准备塑料嵌入的薄片，限制了其功能。在这里，我们从理论和经验上论证了基于反射的FTIRM的发展，作为广泛采用的基于透射式的FTIRM的替代方案，它缩短了样品准备时间并扩大了可成像的样品范围。在这项研究中，成熟的小鼠股骨被塑料包埋，并以4μm的厚度切割纵向切片，用于基于透射式的FTIRM测量。其余的骨块被抛光，以便在紧邻透射区的区域进行基于镜面反射的FTIRM测量。Kramers-Kronig对反射率数据的分析产生了介电响应，由此直接确定了吸收系数。利用薄片的透射谱对反射率得到的吸光度进行了经验验证。在透射式和反射式几何结构中，矿化、碳酸盐取代和胶原交联的光谱指认没有区别，而化学计量/非化学计量磷灰石结晶度参数从基于透射式的1032/1021厘米−1移动到基于反射的数据的1035/1025厘米−1。这种基于反射的FTIRM的理论演示和经验验证消除了对骨骼薄片的需要，并更容易与其他方法直接相关，例如来自同一标本的纳米压痕和定量背向散射电子成像(QBSE)。它为关联骨骼的材料和机械性能提供了一个独特的框架。
Fourier Transform InfraRed Microspectroscopy (FTIRM) is a widely used method for mapping the material properties of bone and other mineralized tissues, including mineralization, crystallinity, carbonate substitution, and collagen cross-linking. This technique is traditionally performed in a transmission-based geometry, which requires the preparation of plastic-embedded thin sections, limiting its functionality. Here, we theoretically and empirically demonstrate the development of reflection-based FTIRM as an alternative to the widely adopted transmission-based FTIRM, which reduces specimen preparation time and broadens the range of specimens that can be imaged. In this study, mature mouse femurs were plastic-embedded and longitudinal sections were cut at a thickness of 4 μm for transmission-based FTIRM measurements. The remaining bone blocks were polished for specular reflectance-based FTIRM measurements on regions immediately adjacent to the transmission sections. Kramers-Kronig analysis of the reflectance data yielded the dielectric response from which the absorption coefficients were directly determined. The reflectance-derived absorbance was validated empirically using the transmission spectra from the thin sections. The spectral assignments for mineralization, carbonate substitution, and collagen cross-linking were indistinguishable in transmission and reflection geometries, while the stoichiometric/non-stoichiometric apatite crystallinity parameter shifted from 1032 / 1021 cm−1 in transmission-based to 1035 / 1025 cm−1 in reflection-based data. This theoretical demonstration and empirical validation of reflection-based FTIRM eliminates the need for thin sections of bone and more readily facilitates direct correlations with other methods such nanoindentation and quantitative backscatter electron imaging (qBSE) from the same specimen. It provides a unique framework for correlating bone’s material and mechanical properties.
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影响因子：
6.2
作者：
Ou-Yang, H;Paschalis, EP;Mendelsohn, R
通讯作者：
Mendelsohn, R
影响因子：
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作者：
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通讯作者：
Boskey, Adele L.
影响因子：
6.2
作者：
Gourion-Arsiquaud, Samuel;Faibish, Dan;Boskey, Adele L.
通讯作者：
Boskey, Adele L.