Hydrogenotrophic microbiota distinguish native Africans from African and European Americans.
Hydrogenotrophic microbiota distinguish native Africans from African and European Americans.
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DOI：
10.1111/j.1758-2229.2012.00334.x
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发表时间：
2012-06
影响因子：
3.3
通讯作者：
Gaskins HR
中科院分区：
文献类型：
--
作者：
Nava GM;Carbonero F;Ou J;Benefiel AC;O'Keefe SJ;Gaskins HR
非洲土著人（NA）对散发性结直肠癌的易感性降低与动物产品的低消费量和结肠甲烷的微生物产量增加有关。在这种情况下，两个氢营养微生物类群引起了人们的兴趣，即利用H2产生甲烷的产甲烷古细菌（MA）和产生硫化氢的硫酸盐还原菌（SRB），后者与结肠的慢性炎症性疾病有关。在本研究中，粪便样本从NA，消耗高的抗性淀粉和低的动物产品的饮食，并从非洲裔美国人（AA）和欧洲裔美国人（EA），都消耗一个典型的西方饮食，进行了检查的遗传多样性和结构的degreea，MA和SRB社区。在一般情况下，更大比例的NA比AA和EA窝藏全方位的目标氢营养基团。16 S rRNA基因和特定功能基因的末端限制性片段长度多态性分析，结合多元统计分析，表明NA比AA和EA含有更多样化和不同的古菌和MA种群。此外，NA窝藏显着不同的SRB种群相比，AA和EA。总之，这些数据与不同氢营养微生物群的饮食选择一致。
Reduced susceptibility to sporadic colorectal cancer in native Africans (NA) is correlated with low consumption of animal products and greater microbial production of colonic methane. In this context, two hydrogenotrophic microbial groups are of interest, methanogenic Archaea (MA) utilizing H2 to produce methane and sulfate-reducing bacteria (SRB) generating hydrogen sulfide, which has been linked with chronic inflammatory disorders of the colon. In the present study, stool samples from NA, consuming a diet high in resistant starch and low in animal products, and from African Americans (AA) and European Americans (EA), both consuming a typical Western diet, were examined for genetic diversity and structure of Archaea, MA and SRB communities. In general, a greater proportion of NA than AA and EA harbored the full range of targeted hydrogenotrophic groups. Terminal restriction fragment length polymorphism analysis of 16S rRNA genes and specific functional genes, combined with multivariate statistical analyses, revealed that NA harbored more diverse and different Archaea and MA populations than AA and EA. Also, NA harbored significantly distinct SRB populations compared to AA and EA. Taken together, these data are consistent with diet selecting for distinct hydrogenotrophic microbiota.
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影响因子：
4.2
作者：
Scanlan PD;Shanahan F;Marchesi JR
通讯作者：
Marchesi JR
影响因子：
9.8
作者：
Levine, J;Ellis, CJ;Levitt, MD
通讯作者：
Levitt, MD
影响因子：
4.2
作者：
GIBSON, GR;CUMMINGS, JH;MACFARLANE, GT
通讯作者：
MACFARLANE, GT
影响因子：
2.6
作者：
Bernalier, A;Rochet, V;Pochart, P
通讯作者：
Pochart, P
影响因子：
11
作者：
Nava, Gerardo M.;Carbonero, Franck;Gaskins, H. Rex
通讯作者：
Gaskins, H. Rex