3D printed microfluidic devices with immunoaffinity monoliths for extraction of preterm birth biomarkers.
3D printed microfluidic devices with immunoaffinity monoliths for extraction of preterm birth biomarkers.
复制标题
3D打印的微流体设备，具有免疫亲和力整体，用于提取早产生物标志物。
DOI：
10.1007/s00216-018-1440-9
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发表时间：
2019-08
影响因子：
4.3
通讯作者：
Woolley AT
中科院分区：
文献类型：
--
作者：
Parker EK;Nielsen AV;Beauchamp MJ;Almughamsi HM;Nielsen JB;Sonker M;Gong H;Nordin GP;Woolley AT
早产（PTB）被定义为怀孕第37周之前出生，每年全球有1500万例早产。目前，没有临床试验来确定PTB风险;然而，在母体血清中发现的一组9种生物标志物对随后的PTB具有预测能力。创建PTB临床诊断的重要一步是设计一种自动化方法来从血清中提取和纯化这些生物标志物。在这里，具有45 μm × 50 μm横截面通道的微流体装置被3D打印，具有内置的聚合窗口，以允许甲基丙烯酸缩水甘油酯整料在通道内进行位点特异性聚合。然后将该整料用作固体支持物以连接用于PTB生物标志物提取的抗体。使用这些功能化的整料，可以从缓冲液和人血清基质中选择性地提取PTB生物标志物铁蛋白。这是在用于免疫亲和提取的3D打印微流体装置中形成整料的第一个证明。值得注意的是，这项工作是开发3D打印微流体临床诊断PTB风险的关键第一步。
Preterm birth (PTB) is defined as birth before the 37th week of pregnancy and results in 15 million early deliveries worldwide every year. Presently, there is no clinical test to determine PTB risk; however, a panel of nine biomarkers found in maternal blood serum has predictive power for a subsequent PTB. A significant step in creating a clinical diagnostic for PTB is designing an automated method to extract and purify these biomarkers from blood serum. Here, microfluidic devices with 45 μm × 50 μm cross-section channels were 3D printed with a built-in polymerization window to allow a glycidyl methacrylate monolith to be site-specifically polymerized within the channel. This monolith was then used as a solid support to attach antibodies for PTB biomarker extraction. Using these functionalized monoliths, it was possible to selectively extract a PTB biomarker, ferritin, from buffer and a human blood serum matrix. This is the first demonstration of monolith formation in a 3D printed microfluidic device for immunoaffinity extraction. Notably, this work is a crucial first step toward developing a 3D printed microfluidic clinical diagnostic for PTB risk.
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影响因子：
9.8
作者：
Esplin MS;Merrell K;Goldenberg R;Lai Y;Iams JD;Mercer B;Spong CY;Miodovnik M;Simhan HN;van Dorsten P;Dombrowski M;Eunice Kennedy Shriver National Institute of Child Health and Human Development Maternal-Fetal Medicine Units Network
通讯作者：
Eunice Kennedy Shriver National Institute of Child Health and Human Development Maternal-Fetal Medicine Units Network
影响因子：
4.3
作者：
Beauchamp MJ;Nordin GP;Woolley AT
通讯作者：
Woolley AT
自动化的微流体设备集成了固相提取，荧光标记和微芯片电泳，以进行早产生物标志物分析。
影响因子：
4.3
作者：
Sahore V;Sonker M;Nielsen AV;Knob R;Kumar S;Woolley AT
通讯作者：
Woolley AT
影响因子：
62.1
作者：
Nge, Pamela N.;Rogers, Chad I.;Woolley, Adam T.
通讯作者：
Woolley, Adam T.
DOI：
10.1126/science.1251816
发表时间：
2014-08-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Romero R;Dey SK;Fisher SJ
通讯作者：
Fisher SJ