Architectural delineation and molecular identification of extracellular matrix in ascidian embryos and larvae.
Architectural delineation and molecular identification of extracellular matrix in ascidian embryos and larvae.
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海鞘胚胎和幼虫细胞外基质的结构描绘和分子鉴定
DOI：
10.1242/bio.026336
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发表时间：
2017-09-15
期刊：
影响因子：
2.4
通讯作者：
Dong B
中科院分区：
文献类型：
--
作者：
Wei J;Wang G;Li X;Ren P;Yu H;Dong B
细胞外基质（ECM）不仅为细胞成分提供必要的物理支架，还引发组织形态发生所必需的关键生化和生物力学信号。在这项研究中，我们利用麦胚凝集素（WGA）染色来表征海鞘胚胎和幼体中的细胞外基质结构。结果显示，在玻璃海鞘胚胎发生过程中存在三种不同的细胞外基质群体：位于胚胎表面的外层、脊索鞘的内层以及由脊索分泌的顶端细胞外基质。为了进一步阐明玻璃海鞘胚胎细胞外基质的精确结构，我们采用了扫描电子显微镜和透射电子显微镜，发现外层膜相对较厚且有短纤维，而脊索鞘中的细胞外基质层不如外层膜厚，但排列更规则；脊索细胞之间的腔是静水的且有粘性。然后，我们利用来自萨氏海鞘胚胎和幼体的RNA测序数据来鉴定细胞外基质基因并获取它们的表达模式。我们将115个单基因鉴定为67个细胞外基质基因，并且77个单基因在不同阶段之间表现出动态表达变化。我们的研究结果揭示了海鞘胚胎发生过程中细胞外基质的结构、分子组成和动态表达谱，可能增进对细胞外基质在脊索动物发育中功能的理解。
总结：这项研究揭示了海鞘胚胎和幼体中细胞外基质的结构、分子组成和动态表达谱，为其在脊索动物发育中的功能提供了线索。
ABSTRACT The extracellular matrix (ECM) not only provides essential physical scaffolding for cellular constituents but also initiates crucial biochemical and biomechanical cues that are required for tissue morphogenesis. In this study, we utilized wheat germ agglutinin (WGA) staining to characterize the ECM architecture in ascidian embryos and larvae. The results showed three distinct populations of ECM presenting in Ciona embryogenesis: the outer layer localized at the surface of embryo, an inner layer of notochord sheath and the apical ECM secreted by the notochord. To further elucidate the precise structure of Ciona embryonic ECM, we employed scanning and transmission electron microscopy, and found that the outer membrane was relatively thick with short fibres, whereas the ECM layer in notochord sheath was not as thick as the outer membrane but more regular arranged; the lumen between notochord cells was hydrostatic and sticky. Then, we used the RNA sequencing data from the embryos and larvae of Ciona savignyi to identify ECM genes and acquire their expression patterns. We identified 115 unigenes as 67 ECM genes, and 77 unigenes showed dynamic expression changes between different stages. Our results reveal the architecture, molecular composition and dynamic expression profile of ECM in ascidian embryogenesis, and may increase understanding of the function of the ECM in chordate development. Summary: This study reveals the architecture, molecular composition and dynamic expression profile of the extracellular matrix in ascidian embryos and larvae, providing clues for its function in chordate development.
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DOI：
10.1083/jcb.201109041
发表时间：
2012-03-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hynes RO
通讯作者：
Hynes RO
影响因子：
--
作者：
Christiaen, Lionel;Wagner, Eileen;Levine, Michael
通讯作者：
Levine, Michael
影响因子：
7.2
作者：
Hynes, Richard O.;Naba, Alexandra
通讯作者：
Naba, Alexandra
影响因子：
4
作者：
Cota, Christina D.;Segade, Fernando;Davidson, Brad
通讯作者：
Davidson, Brad
影响因子：
6.9
作者：
Huxley-Jones, Julie;Robertson, David L.;Boot-Handford, Raymond P.
通讯作者：
Boot-Handford, Raymond P.