A bacteriophage cocktail delivered in feed significantly reduced Salmonella colonization in challenged broiler chickens.
A bacteriophage cocktail delivered in feed significantly reduced Salmonella colonization in challenged broiler chickens.
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DOI：
10.1080/22221751.2023.2217947
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发表时间：
2023-12
影响因子：
13.2
通讯作者：
中科院分区：
文献类型：
--
作者：
非伤寒沙门氏菌属是人类肠胃感染的主要原因，通常通过食用受污染的肉类传播。为了限制沙门氏菌和其他食源性病原体在食物链中的传播，可以在动物生产的饲养或收获前阶段使用噬菌体（噬菌体）治疗。进行该研究以确定在饲料中递送的噬菌体混合物是否能够减少沙门氏菌在实验攻击的鸡中的定殖，并确定最佳噬菌体剂量。总计672只肉鸡被分为六个处理组T1（无噬菌体饲料且未激发）; T2（噬菌体饲料106 PFU/天）; T3（激发组）; T4（噬菌体饲料105 PFU/天并激发）; T5（噬菌体饲料106 PFU/天并激发）;和T6（噬菌体饲料107 PFU/天并激发）。将液体噬菌体混合物添加到糊状饲料中，在整个研究期间可随意获取。到第42天（研究结束日），在从组T4采集的粪便样本中未检出沙门氏菌。从T5组（3/16）和T6组（2/16）的少量畜栏中分离出沙门氏菌，浓度为≤ 4 × 102 CFU/g。相比之下，从T3中7/16个围栏中分离出沙门氏菌，浓度为1.3 × 104 CFU/g。所有三种剂量的噬菌体处理对受攻击鸟类的生长性能具有积极影响，与没有噬菌体饮食的受攻击鸟类相比，体重增加。我们表明，提供沙门氏菌饲料可有效减少鸡中沙门氏菌的定植，我们的研究强调了沙门氏菌为靶向家禽细菌感染提供了一种有前途的工具。
Nontyphoidal Salmonella spp. are a leading cause of human gastrointestinal infections and are commonly transmitted via the consumption of contaminated meat. To limit the spread of Salmonella and other food-borne pathogens in the food chain, bacteriophage (phage) therapy could be used during rearing or pre-harvest stages of animal production. This study was conducted to determine if a phage cocktail delivered in feed is capable of reducing Salmonella colonization in experimentally challenged chickens and to determine the optimal phage dose. A total of 672 broilers were divided into six treatment groups T1 (no phage diet and unchallenged); T2 (phage diet 106 PFU/day); T3 (challenged group); T4 (phage diet 105 PFU/day and challenged); T5 (phage diet 106 PFU/day and challenged); and T6 (phage diet 107 PFU/day and challenged). The liquid phage cocktail was added to mash diet with ad libitum access available throughout the study. By day 42 (the concluding day of the study), no Salmonella was detected in faecal samples collected from group T4. Salmonella was isolated from a small number of pens in groups T5 (3/16) and T6 (2/16) at ∼4 × 102 CFU/g. In comparison, Salmonella was isolated from 7/16 pens in T3 at ∼3 × 104 CFU/g. Phage treatment at all three doses had a positive impact on growth performance in challenged birds with increased weight gains in comparison to challenged birds with no phage diet. We showed delivering phages via feed was effective at reducing Salmonella colonization in chickens and our study highlights phages offer a promising tool to target bacterial infections in poultry.
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影响因子：
1.6
作者：
Castro-Vargas RE;Herrera-Sánchez MP;Rodríguez-Hernández R;Rondón-Barragán IS
通讯作者：
Rondón-Barragán IS
DOI：
10.3390/ph15010058
发表时间：
2022-01-02
期刊：
Pharmaceuticals (Basel, Switzerland)
影响因子：
--
作者：
Thanki AM;Clavijo V;Healy K;Wilkinson RC;Sicheritz-Pontén T;Millard AD;Clokie MRJ
通讯作者：
Clokie MRJ
影响因子：
56.3
作者：
Stanaway, Jeffrey D.;Parisi, Andrea;Crump, John A.
通讯作者：
Crump, John A.
影响因子：
4.4
作者：
Bardina, Carlota;Spricigo, Denis A.;Llagostera, Montserrat
通讯作者：
Llagostera, Montserrat
DOI：
10.3390/ph14040359
发表时间：
2021-04-13
期刊：
Pharmaceuticals (Basel, Switzerland)
影响因子：
--
作者：
Rosner D;Clark J
通讯作者：
Clark J