The Effect of Radiation on the Gut Bacteriome of Aedes albopictus.
The Effect of Radiation on the Gut Bacteriome of Aedes albopictus.
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DOI：
10.3389/fmicb.2021.671699
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发表时间：
2021
影响因子：
5.2
通讯作者：
Bourtzis K
中科院分区：
文献类型：
--
作者：
Zhang D;Chen S;Abd-Alla AMM;Bourtzis K
不育昆虫技术(SIT)已被开发为全区域综合害虫管理方法的组成部分，以控制白纹伊蚊的种群，白纹伊蚊是一种能够传播登革热、寨卡病毒和基孔肯雅病毒的蚊子媒介。SIT成功的关键因素之一是需要高生物质量的不育雄性，一旦释放，它们将能够在野外与野生雄性竞争与野生雌性交配。在昆虫中，肠道细菌体在进化过程中发挥了催化作用，显著影响了昆虫生物学和生态学的几个方面。鉴于肠道相关细菌对宿主的整体生态适合性和生物学质量的重要性，了解辐射对Ae肠道相关细菌群的影响是很有意义的。白纹伊蚊。本研究采用基于16S rRNA基因的下一代测序(NGS)和定量聚合酶链式反应(QPCR)技术，研究了辐射对幼虫期以及1日龄和4日龄雌、雄幼虫肠道相关细菌种类组成和密度水平的影响。年龄、饮食、性别和辐射影响肠道相关细菌群落，其中年龄的影响最大，在蛹、1日龄和4日龄成虫样本中引发细菌多样性和聚集性的显著变化。QPCR分析表明，与所有其他样本相比，雄性样本中气单胞菌的相对密度水平更高，辐射会在特定阶段触发蚊子肠道中气单胞菌和伊利沙伯金酸菌密度水平的增加。我们的结果表明，气单胞菌可能被用作益生菌，以加强保护和性别分离，以支持SIT应用于抗Ae。白纹伊利沙门氏症在受辐射蚊子体内的氧化应激和损伤方面的功能作用还有待进一步研究。
The sterile insect technique (SIT) has been developed as a component of area-wide integrated pest management approaches to control the populations of Aedes albopictus, a mosquito vector capable of transmission of dengue, Zika and chikungunya viruses. One of the key factors for the success of SIT is the requirement of high biological quality sterile males, which upon their release would be able to compete with wild males for matings with wild females in the field. In insects, gut bacteriome have played a catalytic role during evolution significantly affecting several aspects of their biology and ecology. Given the importance of gut-associated bacterial species for the overall ecological fitness and biological quality of their hosts, it is of interest to understand the effects of radiation on the gut-associated bacteriome of Ae. albopictus. In this study, the effect of radiation on the composition and density levels of the gut-associated bacterial species at the pupal stage as well as at 1- and 4-day-old males and females was studied using 16S rRNA gene-based next generation sequencing (NGS) and quantitative PCR (qPCR) approaches. Age, diet, sex, and radiation were shown to affect the gut-associated bacterial communities, with age having the highest impact triggering significant changes on bacterial diversity and clustering among pupae, 1- and 4-day-old adult samples. qPCR analysis revealed that the relative density levels of Aeromonas are higher in male samples compared to all other samples and that the irradiation triggers an increase in the density levels of both Aeromonas and Elizabethkingia in the mosquito gut at specific stages. Our results suggest that Aeromonas could potentially be used as probiotics to enhance protandry and sex separation in support of SIT applications against Ae. albopictus, while the functional role of Elizabethkingia in respect to oxidative stress and damage in irradiated mosquitoes needs further investigation.
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影响因子：
3.7
作者：
Augustinos AA;Kyritsis GA;Papadopoulos NT;Abd-Alla AM;Cáceres C;Bourtzis K
通讯作者：
Bourtzis K
影响因子：
4.2
作者：
Chouaia B;Rossi P;Epis S;Mosca M;Ricci I;Damiani C;Ulissi U;Crotti E;Daffonchio D;Bandi C;Favia G
通讯作者：
Favia G
影响因子：
4.1
作者：
Caputo, Beniamino;Moretti, Riccardo;Calvitti, Maurizio
通讯作者：
Calvitti, Maurizio
影响因子：
3.7
作者：
Balestrino F;Puggioli A;Gilles JR;Bellini R
通讯作者：
Bellini R
影响因子：
5.2
作者：
Chen, Shi;Zhang, Dongjing;Bourtzis, Kostas
通讯作者：
Bourtzis, Kostas