Segmental outflow dynamics in the trabecular meshwork of living mice.
Segmental outflow dynamics in the trabecular meshwork of living mice.
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DOI：
10.1016/j.exer.2022.109285
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发表时间：
2022-12
影响因子：
3.4
通讯作者：
Overby, Darryl R.
中科院分区：
文献类型：
--
作者：
Reina-Torres, Ester;Baptiste, Tiffany M. G.;Overby, Darryl R.
房水不会均匀地通过小梁网（TM）排出，而是遵循非均匀或“节段性”路径。在这项研究中，我们研究了是否节段性流出模式在TM随时间的变化，在活的小鼠，以及这种变化是否受年龄的影响。节段性流出模式标记恒压注入荧光示踪剂微粒到前房的麻醉C57 BL/6 J小鼠在3或8个月的年龄。在不同的时间点输注两种不同的示踪剂颜色，间隔Δt = 0、2、7或14天。在单独的实验中，一种示踪剂在体内输注，而第二种示踪剂在2天后离体输注。使用皮尔逊相关系数r分析两种示踪剂模式之间的空间关系。在3个月大的小鼠中，r存在时间依赖性衰减，在Δt = 0时接近1，在Δt = 14天时接近0。在8个月大的小鼠中，r持续升高14天。考虑到2天内的预期变化后，在离体幼龄小鼠中测量的节段性流出模式与体内测量的节段性流出模式无显著差异。因此，节段性流出模式在TM中不是静态的，而是随着时间的推移而重新分布，在年轻健康小鼠中2周时达到几乎完全丧失相关性。有一个年龄相关的下降率，其中节段性流出模式重新分配在TM。需要进一步的研究来了解控制节段流出的动力因素。
Aqueous humour does not drain uniformly through the trabecular meshwork (TM), but rather follows non-uniform or “segmental” routes. In this study, we examined whether segmental outflow patterns in the TM change over time in living mice and whether such changes are affected by age. Segmental outflow patterns were labelled by constant-pressure infusion of fluorescent tracer microparticles into the anterior chamber of anesthetised C57BL/6J mice at 3 or 8 months of age. Two different tracer colours were infused at separate time points with an interval of Δt = 0, 2, 7 or 14 days. In a separate experiment, one tracer was infused in vivo while the second tracer was infused ex vivo after 2 days. The spatial relationship between the two tracer patterns was analysed using the Pearson’s correlation coefficient, r. In 3-month-old mice, there was a time-dependent decay in r, which was near unity at Δt = 0 and near zero at Δt = 14 days. In 8-month-old mice, r remained elevated for 14 days. Segmental outflow patterns measured in young mice ex vivo were not significantly different from those measured in vivo after accounting for the expected changes over 2 days. Therefore, segmental outflow patterns are not static in the TM but redistribute over time, achieving near complete loss of correlation by 2 weeks in young healthy mice. There is an age-related decline in the rate at which segmental outflow patterns redistribute in the TM. Further research is needed to understand the dynamic factors controlling segmental outflow.
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