Confocal endomicroscopy: instrumentation and medical applications.
Confocal endomicroscopy: instrumentation and medical applications.
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DOI：
10.1007/s10439-011-0426-y
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发表时间：
2012-02
影响因子：
3.8
通讯作者：
Maitland, Kristen C.
中科院分区：
文献类型：
--
作者：
Jabbour, Joey M.;Saldua, Meagan A.;Bixler, Joel N.;Maitland, Kristen C.
光纤技术以及小型化光学和机械技术的进步将共焦内窥镜技术推向了临床领域。这种高分辨率、非侵入性成像技术提供了通过光学切片在显微镜下评估体内组织中的细胞和亚细胞特征的能力。由于许多癌症起源于内窥镜可触及的上皮组织，因此共聚焦内窥镜已被探索通过对组织病理学评估具有重要意义的体内形态特征进行成像，在早期阶段检测可能存在肿瘤的区域。该技术可以实时评估组织，从而可以通过引导活检来提高诊断率。研究和开发不断减小成像探头的整体尺寸，提高图像采集速度，并提高共焦内窥镜的分辨率和视场。技术进步将继续使其能够应用于难以接近的器官和体内更复杂的系统。横向和轴向分辨率分别低至0.5μm和3μm，视场高达800×450μm，物镜和探头总外径分别低至350μm和1.25mm。我们回顾了体内共焦成像的历史发展、内窥镜仪器的演变以及共焦内窥镜的医学应用。
Advances in fiber optic technology and miniaturized optics and mechanics have propelled confocal endomicroscopy into the clinical realm. This high resolution, non-invasive imaging technology provides the ability to microscopically evaluate cellular and sub-cellular features in tissue in vivo by optical sectioning. Because many cancers originate in epithelial tissues accessible by endoscopes, confocal endomicroscopy has been explored to detect regions of possible neoplasia at an earlier stage by imaging morphological features in vivo that are significant in histopathologic evaluation. This technique allows real-time assessment of tissue which may improve diagnostic yield by guiding biopsy. Research and development continues to reduce the overall size of the imaging probe, increase the image acquisition speed, and improve resolution and field of view of confocal endomicroscopes. Technical advances will continue to enable application to less accessible organs and more complex systems in the body. Lateral and axial resolutions down to 0.5 μm and 3 μm, respectively, field of view as large as 800×450 μm, and objective lens and total probe outer diameters down to 350 μm and 1.25 mm, respectively, have been achieved. We provide a review of the historical developments of confocal imaging in vivo, the evolution of endomicroscope instrumentation, and the medical applications of confocal endomicroscopy.
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影响因子：
3.4
作者：
CORCUFF, P;LEVEQUE, JL
通讯作者：
LEVEQUE, JL
影响因子：
4.7
作者：
Gillenwater, Ann;Papadimitrakopoulou, Vali;Richards-Kortum, Rebecca
通讯作者：
Richards-Kortum, Rebecca
影响因子：
3.8
作者：
Boudoux, C;Yun, SH;Tearney, GJ
通讯作者：
Tearney, GJ
影响因子：
46.9
作者：
Becker, A;Hessenius, C;Grötzinger, C
通讯作者：
Grötzinger, C
影响因子：
48
作者：
Barretto, Robert P. J.;Messerschmidt, Bernhard;Schnitzer, Mark J.
通讯作者：
Schnitzer, Mark J.