Biomimetic micropatterned multi-channel nerve guides by templated electrospinning.
Biomimetic micropatterned multi-channel nerve guides by templated electrospinning.
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DOI：
10.1002/bit.24412
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发表时间：
2012-06
影响因子：
3.8
通讯作者：
Wang, Yadong
中科院分区：
文献类型：
--
作者：
Jeffries, Eric M.;Wang, Yadong
该报告描述了一种在三维静电纺丝结构中制造微通道的新方法。这些关键特征用于模仿天然神经中发现的束状结构和纤维细胞外基质。电纺纤维和多通道结构神经导管因其在神经修复中的有益作用而受到越来越多的关注。然而，据我们所知，这是第一份将两者结合起来的指南报告。与圆柱形导轨相比，多个平行通道可提供更多数量的限定路径和更大的表面积。此外，电纺纤维的纤维性质比实壁结构具有更好的质量传输能力。灵活的制造方案允许定制神经导管参数，例如 33-176μm 的通道直径和各种壁厚。通过光学和电子显微镜图像评估通道和纤维结构。几何计算估计这些导轨的孔隙率超过 85%，而通道的孔隙率为 16% 或更少。雪旺细胞的体外培养表明细胞浸润到纤维间迁移受限的通道中。最后，整个指南中的细胞增殖和存活表明该设计值得未来的体内检查。
This report describes a new approach for fabricating micro-channels within three-dimensional electrospun constructs. These key features serve to mimic the fascicular architecture and fibrous extracellular matrix found in native nerve. Both electrospun fibers and multi-channeled structure nerve guides have become areas of increasing interest for their beneficial roles in nerve repair. However, to the best of our knowledge, this is the first report of a guide that incorporates both. Multiple parallel channels provide a greater number of defined paths and increased surface area compared to cylindrical guides. Additionally, the fibrous nature of electrospun fibers permits better mass transport than solid-walled constructs. The flexible fabrication scheme allows tailoring of nerve guide parameters such as channel diameters ranging from 33-176μm and various wall thicknesses. Channel and fiber structures were assessed by optical and electron microscope images. Geometric calculations estimated a porosity of over 85% for these guides with 16% or less from the channels. In vitro culture with Schwann cells demonstrated cellular infiltration into channels with restricted migration between fibers. Finally, cell proliferation and survival throughout the guide indicates that this design warrants future in vivo examination.
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DOI：
10.1002/jbm.b.30510
发表时间：
2006-10-01
影响因子：
3.4
作者：
Jiang, Hongliang;Hu, Yingqian;Zhu, Kangjie
通讯作者：
Zhu, Kangjie
影响因子：
4.8
作者：
de Ruiter, Godard C.;Spinner, Robert J.;Windebank, Anthony J.
通讯作者：
Windebank, Anthony J.
DOI：
10.1097/01.jnen.0000171650.94341.46
发表时间：
2005-07-01
影响因子：
3.2
作者：
Chen, YY;McDonald, D;Zochodne, DW
通讯作者：
Zochodne, DW
影响因子：
4.9
作者：
George, Paul M.;Saigal, Rajiv;Kohane, Daniel S.
通讯作者：
Kohane, Daniel S.
影响因子：
4.9
作者：
Ao, Q;Wang, AJ;Zhang, XF
通讯作者：
Zhang, XF