The genome of the stable fly, Stomoxys calcitrans, reveals potential mechanisms underlying reproduction, host interactions, and novel targets for pest control.
The genome of the stable fly, Stomoxys calcitrans, reveals potential mechanisms underlying reproduction, host interactions, and novel targets for pest control.
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DOI：
10.1186/s12915-021-00975-9
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发表时间：
2021-03-10
期刊：
影响因子：
5.4
通讯作者：
Benoit JB
中科院分区：
文献类型：
--
作者：
Olafson PU;Aksoy S;Attardo GM;Buckmeier G;Chen X;Coates CJ;Davis M;Dykema J;Emrich SJ;Friedrich M;Holmes CJ;Ioannidis P;Jansen EN;Jennings EC;Lawson D;Martinson EO;Maslen GL;Meisel RP;Murphy TD;Nayduch D;Nelson DR;Oyen KJ;Raszick TJ;Ribeiro JMC;Robertson HM;Rosendale AJ;Sackton TB;Saelao P;Swiger SL;Sze SH;Tarone AM;Taylor DB;Warren WC;Waterhouse RM;Weirauch MT;Werren JH;Wilson RK;Zdobnov EM;Benoit JB
Stomoxys calcitrans是牲畜的主要吸血害虫，几乎遍布全球，仅在美国每年就造成超过20亿美元的控制和产品损失成本。对这些蝇的控制仅限于增加卫生管理措施和应用杀虫剂抑制幼虫阶段。很少有遗传和分子资源可用于帮助开发控制稳定苍蝇的新方法。这项研究通过利用高质量的基因组测序和针对多个发育阶段和组织的RNA-Seq分析的组合来检查稳定的苍蝇生物学。结合，1600个基因被手动策划，以表征与稳定的苍蝇繁殖，载体宿主相互作用，宿主-微生物动力学和推定的控制目标相关的遗传特征。最值得注意的是与生殖相关的基因的表征和扩展的基因家族与视觉，化学感觉，免疫和代谢解毒途径的功能关联的鉴定。雄性稳定果蝇基因组的组合测序，组装和管理，然后进行RNA-Seq和下游分析，为了解这种重要害虫的生物学提供了必要的见解。这些资源和新的数据将为扩大控制稳定苍蝇侵扰的工具提供基础。Stomoxys的密切关系，以其他血液喂养（角蝇和Glossina）和非血液喂养苍蝇（家蝇，medflies，果蝇）将有助于理解的进化过程与发展之间的Cyclorrhapha血液喂养。在线版本包含补充材料，可通过10.1186/s12915-021-00975-9获得。
The stable fly, Stomoxys calcitrans, is a major blood-feeding pest of livestock that has near worldwide distribution, causing an annual cost of over $2 billion for control and product loss in the USA alone. Control of these flies has been limited to increased sanitary management practices and insecticide application for suppressing larval stages. Few genetic and molecular resources are available to help in developing novel methods for controlling stable flies. This study examines stable fly biology by utilizing a combination of high-quality genome sequencing and RNA-Seq analyses targeting multiple developmental stages and tissues. In conjunction, 1600 genes were manually curated to characterize genetic features related to stable fly reproduction, vector host interactions, host-microbe dynamics, and putative targets for control. Most notable was characterization of genes associated with reproduction and identification of expanded gene families with functional associations to vision, chemosensation, immunity, and metabolic detoxification pathways. The combined sequencing, assembly, and curation of the male stable fly genome followed by RNA-Seq and downstream analyses provide insights necessary to understand the biology of this important pest. These resources and new data will provide the groundwork for expanding the tools available to control stable fly infestations. The close relationship of Stomoxys to other blood-feeding (horn flies and Glossina) and non-blood-feeding flies (house flies, medflies, Drosophila) will facilitate understanding of the evolutionary processes associated with development of blood feeding among the Cyclorrhapha. The online version contains supplementary material available at 10.1186/s12915-021-00975-9.
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影响因子：
2.6
作者：
Olafson PU
通讯作者：
Olafson PU
影响因子：
12.3
作者：
Barribeau SM;Sadd BM;du Plessis L;Brown MJ;Buechel SD;Cappelle K;Carolan JC;Christiaens O;Colgan TJ;Erler S;Evans J;Helbing S;Karaus E;Lattorff HM;Marxer M;Meeus I;Näpflin K;Niu J;Schmid-Hempel R;Smagghe G;Waterhouse RM;Yu N;Zdobnov EM;Schmid-Hempel P
通讯作者：
Schmid-Hempel P
影响因子：
5.7
作者：
Barbagallo B;Garrity PA
通讯作者：
Garrity PA
影响因子：
30.8
作者：
Akman, L;Yamashita, A;Aksoy, S
通讯作者：
Aksoy, S
影响因子：
3.4
作者：
Ahmed MZ;Breinholt JW;Kawahara AY
通讯作者：
Kawahara AY