Tissue-specific transcriptome responses to Fusarium head blight and Fusarium root rot.
Tissue-specific transcriptome responses to Fusarium head blight and Fusarium root rot.
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DOI：
10.3389/fpls.2022.1025161
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
镰刀菌头枯病和镰刀菌根腐病是由镰刀菌属真菌引起的小粒谷物的重要病害。虽然宿主对FHB的反应已经进行了广泛的研究，但对FRR的反应知之甚少，并且FHB和FRR的转录组反应尚未进行彻底的比较。二穗短柄草（Brachypodium distachyon，Bd）是研究寄主对FHB和FRR反应的有效模型。在本研究中，Bd对F.研究了禾谷镰刀菌（Graminearum，Fg）对小麦穗部和根部的侵染情况。在早期感染期间对Bd FHB和FRR两者进行RNA-seq分析。此外，对Fg的体外样品进行RNA-seq分析，以与植物中的Fg基因表达进行比较。差异基因表达和基因列表富集分析用于比较Bd和Fg中的FHB和FRR转录组响应。使用RT-qPCR确认所选基因的差异表达。大多数与受体信号，细胞壁修饰，氧化应激代谢，细胞分裂素和生长素的生物合成和信号基因相关的基因通常在FHB中上调或在FRR中下调。相反，参与茉莉酸和乙烯的生物合成和信号传导，抗菌素生产的Bd基因在两种组织中响应感染的差异表达相似。预测的Fg效应与相同的感染材料的转录组分析显示，核心组织独立的基因，包括细胞壁降解酶和DON生产的基因簇，但也有几个组织依赖的基因，包括金镰菌素生产和角质降解的表达升高。这一证据表明，Fg调节其转录组到同一宿主的不同组织。
Fusarium head blight (FHB) and Fusarium root rot (FRR) are important diseases of small-grain cereals caused by Fusarium species. While host response to FHB has been subject to extensive study, very little is known about response to FRR and the transcriptome responses of FHB and FRR have not been thoroughly compared. Brachypodium distachyon (Bd) is an effective model for investigating host responses to both FHB and FRR. In this study the transcriptome response of Bd to F. graminearum (Fg) infection of heads and roots was investigated. An RNA-seq analysis was performed on both Bd FHB and FRR during the early infection. Additionally, an RNA-seq analysis was performed on in vitro samples of Fg for comparison with Fg gene expression in planta. Differential gene expression and gene-list enrichment analyses were used to compare FHB and FRR transcriptome responses in both Bd and Fg. Differential expression of selected genes was confirmed using RT-qPCR. Most genes associated with receptor signalling, cell-wall modification, oxidative stress metabolism, and cytokinin and auxin biosynthesis and signalling genes were generally upregulated in FHB or were downregulated in FRR. In contrast, Bd genes involved in jasmonic acid and ethylene biosynthesis and signalling, and antimicrobial production were similarly differentially expressed in both tissues in response to infection. A transcriptome analysis of predicted Fg effectors with the same infected material revealed elevated expression of core tissue-independent genes including cell-wall degradation enzymes and the gene cluster for DON production but also several tissue-dependent genes including those for aurofusarin production and cutin degradation. This evidence suggests that Fg modulates its transcriptome to different tissues of the same host.
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影响因子：
14.9
作者：
Gene Ontology Consortium
通讯作者：
Gene Ontology Consortium
影响因子：
4.9
作者：
Brown NA;Evans J;Mead A;Hammond-Kosack KE
通讯作者：
Hammond-Kosack KE
影响因子：
3
作者：
Alexander, Nancy J.;McCormick, Susan P.;Proctor, Robert H.
通讯作者：
Proctor, Robert H.
影响因子：
4.4
作者：
Buerstmayr M;Wagner C;Nosenko T;Omony J;Steiner B;Nussbaumer T;Mayer KFX;Buerstmayr H
通讯作者：
Buerstmayr H
影响因子：
3.7
作者：
Brown NA;Antoniw J;Hammond-Kosack KE
通讯作者：
Hammond-Kosack KE