Macrophage-melanocyte heterokaryons. I. Preparation and properties.
Macrophage-melanocyte heterokaryons. I. Preparation and properties.
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巨噬细胞螺旋细胞异源性。 I.制备和特性。
DOI：
10.1084/jem.131.5.981
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发表时间：
1970-05-01
影响因子：
15.3
通讯作者：
COHN, Z
中科院分区：
文献类型：
--
作者：
GORDON, S;COHN, Z
通过病毒诱导的细胞在玻璃表面上的融合，获得了高产量的小鼠巨噬细胞-黑素细胞异核体和巨噬细胞-巨噬细胞同核体。融合后有一个显着的重组细胞结构的秋水仙胺敏感的过程。异核体分离通过使用差异胰蛋白酶消化和许多进行分裂，形成黑素细胞样杂种。巨噬细胞对硫酸葡聚糖的选择性摄取可作为鉴定杂交种的有用细胞质标记。在异核体中，许多特征性的巨噬细胞特性发生了改变。融合后1小时内，巨噬细胞核肿胀，核仁更加突出，核RNA合成增加。融合后3小时，在先前休眠的巨噬细胞核中发生DNA合成波。在异核体和同核体中检查典型巨噬细胞标志物的命运。巨噬细胞同核体继续表现出积极的吞噬致敏红细胞，而这种能力是不可逆的异核体。异核体吞噬活性的丧失以指数速率发生，并被高浓度的小牛血清加速。另一种巨噬细胞表面标志物，二价阳离子依赖性腺苷三磷酸酶（ATP酶），可以证明异核体的组织化学。融合后不久，它出现在离散的区域，但它变得更加分散，并在一天内消失。酸性磷酸酶阳性的次级溶酶体和可伸缩的脂滴从异核体中消失，但继续在巨噬细胞同源核中积累。这些观察结果表明，典型的巨噬细胞特性停止在异核体中表达，并且黑素细胞功能大概在异核体和杂交体中占主导地位。
High yields of mouse macrophage-melanocyte heterokaryons and macrophage-macrophage homokaryons were obtained through the virus-induced fusion of cells spread on a glass surface. After fusion there was a striking reorganization of cellular architecture by means of a colcemid-sensitive process. Heterokaryons were isolated through the use of differential trypsinization and many underwent division to form melanocyte-like hybrids. The selective uptake of dextran sulfate by macrophages served as a useful cytoplasmic marker in identifying hybrids. Many characteristic macrophage properties were altered in the heterokaryons. Within an hour of fusion macrophage nuclei became swollen, nucleoli were more prominent, and increased nuclear RNA synthesis occurred. 3 hr after fusion, a wave of DNA synthesis took place in the previously dormant macrophage nuclei. The fate of typical macrophage markers was examined in both heterokaryons and homokaryons. Macrophage homokaryons continued to exhibit active phagocytosis of sensitized erythrocytes, whereas this capacity was lost irreversibly in heterokaryons. The loss of phagocytic activity of heterokaryons occurred at an exponential rate and was accelerated by high concentrations of calf serum. Another macrophage surface marker, a divalent cation-dependent adenosine triphosphatase (ATPase), could be demonstrated histochemically on heterokaryons. Shortly after fusion, it was present in discrete regions, but it became more diffuse and disappeared within a day. Acid phosphatase-positive secondary lysosomes and retractile lipid droplets disappeared from heterokaryons but continued to accumulate in macrophage homokaryons. These observations indicate that typical macrophage properties cease to be expressed in heterokaryons, and melanocyte functions presumably predominate in heterokaryons and hybrids.
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单核吞噬细胞的起源和动力学。
影响因子：
15.3
作者：
van Furth, R;Cohn, Z A
通讯作者：
Cohn, Z A
影响因子：
7.8
作者：
HOLMES, KV;CHOPPIN, PW
通讯作者：
CHOPPIN, PW
影响因子：
5.6
作者：
DAVIDSON, R;EPHRUSSI, B;YAMAMOTO, K
通讯作者：
YAMAMOTO, K
影响因子：
15.3
作者：
COHN, ZA;BENSON, B
通讯作者：
BENSON, B
DOI：
10.3181/00379727-98-24092
发表时间：
1958-01-01
影响因子：
--
作者：
CHOPPIN, PW;OSTERHOUT, S;TAMM, I
通讯作者：
TAMM, I