Improved methods for MRI-compatible implants in nonhuman primates.
Improved methods for MRI-compatible implants in nonhuman primates.
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DOI：
10.1016/j.jneumeth.2018.09.013
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发表时间：
2018-10-01
影响因子：
3
通讯作者：
Schmid MC
中科院分区：
文献类型：
--
作者：
Ortiz-Rios M;Haag M;Balezeau F;Frey S;Thiele A;Murphy K;Schmid MC
我们创建了定制的羟基磷灰石涂层PEEK植入物，以促进骨整合。用陶瓷螺钉植入头柱和腔室，并皮下闭合手术切口。我们通过使用保护伤口边缘的头帽防止动物在手术后采摘和抓挠。植入物与颅骨结合，一年后仍然坚固，没有肉芽组织生长。MRI信号和对比度噪声比改善NHP植入我们的新方法。神经科学家通常在电生理学和功能性磁共振成像（fMRI）过程中使用永久植入的头柱来稳定非人类灵长类动物（NHP）的头部。在这里，我们提出了改进的方法，MRI兼容的植入物，而不使用丙烯酸头稳定在NHP。MRI用于获得NHP颅骨的3D重建，NHP颅骨用于通过对与骨的交叉进行建模来创建定制的植入物。植入物由PEEK通过计算机数控加工制成，并涂有羟基磷灰石以促进骨整合。在外科手术中，植入物用陶瓷螺钉固定在颅骨上，同时皮瓣被拉到植入物上并在皮下闭合。与传统的丙烯酸植入物相比，用我们的方法植入的动物的血氧水平依赖性（BOLD）fMRI信号的质量得到改善。此外，植入物与颅骨结合良好，保持坚固超过一年，并且皮肤边缘周围没有肉芽组织。先前对NHP植入物的改进（Chen等人，2017; McAndrew等人，2012; Mulliken等人，2015; Overton等人，2017）缺乏fMRI兼容性，因为它们依赖于钛头柱和/或钛螺钉。因此，大多数功能性磁共振成像研究在NHP今天仍然依赖于使用丙烯酸基头柱的稳定和使用对比增强剂，以改善MRI信号。我们的方法保留了fMRI的兼容性，并导致BOLD信号的可测量的改善，而无需使用对比增强剂。此外，我们植入物的长期稳定性对神经科学研究中NHP的健康有积极的贡献。
We created custom PEEK implants coated with hydroxylapatite to promote osseointegration. Headposts and chambers were implanted with ceramic screws and the surgical incision was closed subcutaneously. We prevented the animal from picking and scratching after surgery by using a head cap that protects the wound margin. Implants integrated with the skull and remained robust after a year with no growth of granulation tissue. MRI signal and contrast-to-noise ratio improved in NHPs implanted with our new methods. Neuroscientists commonly use permanently implanted headposts to stabilize the head of nonhuman primates (NHPs) during electrophysiology and functional magnetic resonance imaging (fMRI). Here, we present improved methodology for MRI-compatible implants without the use of acrylic for head stabilization in NHPs. MRI is used to obtain a 3D-reconstruction of NHP skulls, which are used to create customized implants by modeling intersections with the bone. Implants are manufactured from PEEK using computer numerical control machining and coated with hydroxyapatite to promote osseointegration. Surgically, implants are attached to the skull with ceramic screws, while the skin flap is pulled over the implant and closed subcutaneously. Quality of blood oxygen level dependent (BOLD) fMRI signal is improved in animals implanted with our method as compared to traditional acrylic implants. Additionally, implants are well-integrated with the skull, remain robust for more than a year and without granulation tissue around the skin margin. Previous improvements on NHP implants (Chen et al., 2017; McAndrew et al., 2012; Mulliken et al., 2015; Overton et al., 2017) lacked fMRI-compatibility, as they relied on titanium headposts and/or titanium screws. Thus, most fMRI studies in NHPs today still rely on the use of acrylic-based headposts for stabilization and the use of contrast-enhanced agents to improve MRI signal. Our method preserves fMRI-compatibility and results in measurable improvement in BOLD signal without the use of contrast-enhanced agents. Furthermore, the long-term stability of our implants contributes positively to the wellbeing of NHPs in neuroscience research.
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影响因子：
3
作者：
McAndrew RM;Lingo VanGilder JL;Naufel SN;Helms Tillery SI
通讯作者：
Helms Tillery SI
早期视觉皮层中额叶信号的自下而上的门控。
DOI：
10.1126/science.1153276
发表时间：
2008-07-18
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ekstrom LB;Roelfsema PR;Arsenault JT;Bonmassar G;Vanduffel W
通讯作者：
Vanduffel W
影响因子：
3
作者：
Murnane, Kevin Sean;Howell, Leonard Lee
通讯作者：
Howell, Leonard Lee
当前提高 PEEK 生物活性的策略
影响因子：
5.6
作者：
Ma R;Tang T
通讯作者：
Tang T
影响因子：
5.7
作者：
Janssens, T.;Keil, B.;Farivar, R.;McNab, J. A.;Polimeni, J. R.;Gerits, A.;Arsenault, J. T.;Wald, L. L.;Vanduffel, W.
通讯作者：
Vanduffel, W.