Induction of anti-viral immune responses by immunization with recombinant-DNA encoded avian coronavirus nucleocapsid protein.
Induction of anti-viral immune responses by immunization with recombinant-DNA encoded avian coronavirus nucleocapsid protein.
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DOI：
10.1016/0264-410x(92)90028-i
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发表时间：
1992
期刊：
影响因子：
5.5
通讯作者：
Hensen EJ
中科院分区：
文献类型：
--
作者：
Boots AM;Benaissa-Trouw BJ;Hesselink W;Rijke E;Schrier C;Hensen EJ
用重组DNA表达产物研究了传染性支气管炎病毒(IBV)核衣壳蛋白的免疫应答。在小鼠中，这种核衣壳蛋白免疫后，诱导了淋巴细胞增殖反应和对IBV的迟发性超敏反应。接下来，我们研究了表达的核衣壳蛋白在诱导鸡对IBV的保护性免疫反应中的作用。给鸡注射核衣壳蛋白，然后用灭活的IBV M41株加强免疫。血液单个核细胞的增殖反应与平均血凝抑制和病毒中和滴度增加相对应。最后，观察到对IBV活体攻击的气管保护作用增强。这些结果表明，传染性支气管炎病毒核衣壳蛋白是小鼠和鸡免疫识别的相关靶点。
Immune responses to the infectious bronchitis virus (IBV) nucleocapsid protein were studied using a recombinant-DNA expression product. In mice, a lymphocyte proliferative response and a delayed-type hypersensitivity reaction to IBV were induced upon immunization with this nucleocapsid protein. Next, we studied the role of the expressed nucleocapsid protein in induction of a protective immune response to IBV in chickens. Chickens were primed with nucleocapsid protein and subsequently boosted with inactivated IBV, strain M41. Proliferative responses of blood mononuclear cells corresponded with increased mean haemagglutination inhibition and virus neutralization titres. Finally, an increased tracheal protection against challenge with live IBV was observed. These results indicate that infectious bronchitis virus nucleocapsid protein is a relevant target for immune recognition in both the mouse and the chicken.
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