Abscission Couples Cell Division to Embryonic Stem Cell Fate.
Abscission Couples Cell Division to Embryonic Stem Cell Fate.
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DOI：
10.1016/j.devcel.2020.09.001
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发表时间：
2020-10-26
影响因子：
11.8
通讯作者：
Paluch EK
中科院分区：
文献类型：
--
作者：
Chaigne A;Labouesse C;White IJ;Agnew M;Hannezo E;Chalut KJ;Paluch EK
细胞命运转变是发育和稳态的关键。因此，了解控制命运转变的细胞机制至关重要。细胞分裂与许多干细胞类型的命运决定有关，包括神经元和上皮祖细胞。在其他干细胞中，如胚胎干细胞（ES），分裂的作用仍不清楚。在这里，我们表明，退出幼稚多能性小鼠ES细胞一般发生在分裂后。我们进一步表明，退出时间是密切相关的姐妹细胞，分裂后很长一段时间内保持连接的细胞质桥，和桥分裂逐渐加速细胞退出幼稚多能性。最后，干扰脱落会损害幼稚多能性的退出，而人为诱导脱落会加速它。总而言之，我们的数据表明，分裂机制的转换导致更快的脱落，从而调节多能性的退出。我们的研究确定了细胞分裂是一个关键的细胞过程耦合细胞分裂的命运转变。小鼠胚胎干细胞在有丝分裂后退出幼稚多能性幼稚胚胎干细胞表现出缓慢的分裂并保持通过桥连接退出幼稚多能性的细胞表现出更快的分裂加速分裂促进退出幼稚多能性细胞分裂已经涉及许多干细胞的命运决定。Chaigne等人显示小鼠胚胎干细胞在细胞分裂后退出幼稚多能性。幼稚姐妹细胞在分裂后通过细胞质桥保持连接，并且桥的断裂促进幼稚多能性退出。
Cell fate transitions are key to development and homeostasis. It is thus essential to understand the cellular mechanisms controlling fate transitions. Cell division has been implicated in fate decisions in many stem cell types, including neuronal and epithelial progenitors. In other stem cells, such as embryonic stem (ES) cells, the role of division remains unclear. Here, we show that exit from naive pluripotency in mouse ES cells generally occurs after a division. We further show that exit timing is strongly correlated between sister cells, which remain connected by cytoplasmic bridges long after division, and that bridge abscission progressively accelerates as cells exit naive pluripotency. Finally, interfering with abscission impairs naive pluripotency exit, and artificially inducing abscission accelerates it. Altogether, our data indicate that a switch in the division machinery leading to faster abscission regulates pluripotency exit. Our study identifies abscission as a key cellular process coupling cell division to fate transitions. Mouse embryonic stem cells exit naive pluripotency after mitosis Naive embryonic stem cells display slow abscission and remain connected by bridges Cells exiting naive pluripotency display faster abscission Accelerating abscission facilitates exit from naive pluripotency Cell division has been implicated in fate decisions in many stem cells. Chaigne et al. show that mouse embryonic stem cells exit naive pluripotency after cell division. Naive sister cells remain connected by cytoplasmic bridges after division, and bridge abscission promotes naive pluripotency exit.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
56.9
作者：
Lafaurie-Janvore, Julie;Maiuri, Paolo;Piel, Matthieu
通讯作者：
Piel, Matthieu
影响因子：
4.3
作者：
Gershony, Ofir;Pe'er, Tal;Tzur, Amit
通讯作者：
Tzur, Amit
影响因子：
56.9
作者：
Carlton, Jez G.;Martin-Serrano, Juan
通讯作者：
Martin-Serrano, Juan
影响因子：
0.7
作者：
David, Charles N.
通讯作者：
David, Charles N.