Secretor genotype (FUT2 gene) is strongly associated with the composition of Bifidobacteria in the human intestine.
Secretor genotype (FUT2 gene) is strongly associated with the composition of Bifidobacteria in the human intestine.
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DOI：
10.1371/journal.pone.0020113
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Mättö J
中科院分区：
文献类型：
--
作者：
Wacklin P;Mäkivuokko H;Alakulppi N;Nikkilä J;Tenkanen H;Räbinä J;Partanen J;Aranko K;Mättö J
肠道微生物群在人类健康中起着重要作用，其组成由多种因素决定，如饮食和宿主基因型。然而，到目前为止，仍然不知道哪些宿主基因是微生物群组成的决定因素。我们研究了71名健康人粪便样本中优势细菌和双歧杆菌的多样性和丰度。在该队列中，14名为非分泌型个体，而分泌型个体为分泌型个体。分泌状态由ABH和刘易斯组织血型抗原在肠粘液和其他分泌物中的表达来定义。由FUT 2基因编码的岩藻糖基转移酶2决定。FUT 2基因无义突变导致的无功能酶导致非分泌型表型。采用PCR-DGGE和qPCR方法进行肠道菌群分析。DGGE图谱的主成分分析表明，非分泌型个体的样品在分泌型样品中形成了一个单独的簇。此外，与来自分泌型个体的样品相比，来自非分泌型个体的样品中的双歧杆菌多样性（p <0.0001）、丰富度（p<0.0003）和丰度（p<0.05）显著降低。非分泌型个体缺乏或很少被与B相关的几种基因型定殖。bifidum，B.我和B。catenulatum/pseudocatenulatum。PCR-DGGE检测到的优势菌丰度（p<0.04）在非分泌型个体中高于分泌型个体。我们发现，人类肠道菌群的多样性和组成与组织血型ABH分泌/非分泌状态密切相关，因此似乎是肠道微生物群组成的宿主遗传决定因素之一。这种关联可以通过分泌型和非分泌型个体在粘膜中ABH和刘易斯聚糖表位表达的差异来解释。
Intestinal microbiota plays an important role in human health, and its composition is determined by several factors, such as diet and host genotype. However, thus far it has remained unknown which host genes are determinants for the microbiota composition. We studied the diversity and abundance of dominant bacteria and bifidobacteria from the faecal samples of 71 healthy individuals. In this cohort, 14 were non-secretor individuals and the remainders were secretors. The secretor status is defined by the expression of the ABH and Lewis histo-blood group antigens in the intestinal mucus and other secretions. It is determined by fucosyltransferase 2 enzyme, encoded by the FUT2 gene. Non-functional enzyme resulting from a nonsense mutation in the FUT2 gene leads to the non-secretor phenotype. PCR-DGGE and qPCR methods were applied for the intestinal microbiota analysis. Principal component analysis of bifidobacterial DGGE profiles showed that the samples of non-secretor individuals formed a separate cluster within the secretor samples. Moreover, bifidobacterial diversity (p<0.0001), richness (p<0.0003), and abundance (p<0.05) were significantly reduced in the samples from the non-secretor individuals as compared with those from the secretor individuals. The non-secretor individuals lacked, or were rarely colonized by, several genotypes related to B. bifidum, B. adolescentis and B. catenulatum/pseudocatenulatum. In contrast to bifidobacteria, several bacterial genotypes were more common and the richness (p<0.04) of dominant bacteria as detected by PCR-DGGE was higher in the non-secretor individuals than in the secretor individuals. We showed that the diversity and composition of the human bifidobacterial population is strongly associated with the histo-blood group ABH secretor/non-secretor status, which consequently appears to be one of the host genetic determinants for the composition of the intestinal microbiota. This association can be explained by the difference between the secretor and non-secretor individuals in their expression of ABH and Lewis glycan epitopes in the mucosa.
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