Precise quantification of silica and ceria nanoparticle uptake revealed by 3D fluorescence microscopy.
Precise quantification of silica and ceria nanoparticle uptake revealed by 3D fluorescence microscopy.
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DOI：
10.3762/bjnano.5.173
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发表时间：
2014
影响因子：
3.1
通讯作者：
Bräuchle C
中科院分区：
文献类型：
--
作者：
Torrano AA;Bräuchle C
Particle_in_Cell-3D是量化纳米颗粒细胞摄取的强大方法。它结合了共聚焦荧光显微镜的优点和快速精确的半自动图像分析。在这项工作中，我们提出了如何将这种方法应用于研究310 nm二氧化硅纳米粒子对人血管内皮细胞（HUVEC）的影响，比较来自宫颈癌（HeLa）的癌细胞系。细胞内的二氧化硅纳米粒子的绝对数量在第一个24小时内进行了测定，并显示是细胞类型依赖性的。作为第二个案例研究，Particle_in_Cell-3D用于评估与人微血管内皮细胞（HMEC-1）相互作用的8 nm和30 nm二氧化铈纳米颗粒的摄取动力学。这些小的纳米颗粒在生物介质中形成聚集体，与细胞有效接触的颗粒的平均直径分别为417 nm和316 nm。一个显着的颗粒大小依赖性的影响后，观察到48小时的相互作用，和细胞内颗粒的数量是大于四倍的316 nm的团聚体。有趣的是，我们的结果表明，对于两种粒径，细胞内纳米颗粒的最大剂量在约24小时。这种有趣和不寻常的摄取行为的原因之一可能是细胞分裂。
Particle_in_Cell-3D is a powerful method to quantify the cellular uptake of nanoparticles. It combines the advantages of confocal fluorescence microscopy with fast and precise semi-automatic image analysis. In this work we present how this method was applied to investigate the impact of 310 nm silica nanoparticles on human vascular endothelial cells (HUVEC) in comparison to a cancer cell line derived from the cervix carcinoma (HeLa). The absolute number of intracellular silica nanoparticles within the first 24 h was determined and shown to be cell type-dependent. As a second case study, Particle_in_Cell-3D was used to assess the uptake kinetics of 8 nm and 30 nm ceria nanoparticles interacting with human microvascular endothelial cells (HMEC-1). These small nanoparticles formed agglomerates in biological medium, and the particles that were in effective contact with cells had a mean diameter of 417 nm and 316 nm, respectively. A significant particle size-dependent effect was observed after 48 h of interaction, and the number of intracellular particles was more than four times larger for the 316 nm agglomerates. Interestingly, our results show that for both particle sizes there is a maximum dose of intracellular nanoparticles at about 24 h. One of the causes for such an interesting and unusual uptake behavior could be cell division.
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影响因子：
13.3
作者：
Andersson, Per Ola;Lejon, Christian;Osterlund, Lars
通讯作者：
Osterlund, Lars
影响因子：
4.1
作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
Handley J
影响因子：
17.1
作者：
Asati A;Santra S;Kaittanis C;Perez JM
通讯作者：
Perez JM