The Pentatricopeptide Repeat Gene Family in Salvia miltiorrhiza: Genome-Wide Characterization and Expression Analysis.
The Pentatricopeptide Repeat Gene Family in Salvia miltiorrhiza: Genome-Wide Characterization and Expression Analysis.
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丹参中五肽重复基因家族：全基因组表征和表达分析
DOI：
10.3390/molecules23061364
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发表时间：
2018-06-06
期刊：
影响因子：
--
通讯作者：
Lu S
中科院分区：
文献类型：
--
作者：
Li H;Li C;Deng Y;Jiang X;Lu S
五肽重复序列（PPR）基因家族是植物中最大的基因家族之一，在转录后调控中起着重要作用。本研究结合全基因组测序和转录组技术，系统地研究了丹参的PPR，丹参是一种著名的中药材料，也是药用植物研究的新兴模式系统。在562个已鉴定的SmPPRs中，299个属于P亚家族，而其他的属于PLS亚家族。大多数SmPPR只有一个外显子，并且定位于线粒体或叶绿体中。多达546个SmPPR在至少一种组织中表达，并显示出差异表达模式，这表明它们可能在S.丹参。有349个SmPPR对水杨酸敏感，183个SmPPR对酵母抽提物和Ag+敏感，表明这些基因可能参与了S.丹参防御胁迫和次生代谢。此外，23个水杨酸响应SmPPR与酚酸生物合成酶基因共表达，而16个酵母提取物和Ag+响应SmPPR与丹参酮生物合成酶基因共表达。两个SmPPR与酚酸和丹参酮生物合成酶基因共表达。这些结果为进一步研究PPRs在S.丹参。
The pentatricopeptide repeat (PPR) gene family is one of the largest gene families in plants and plays important roles in posttranscriptional regulation. In this study, we combined whole genome sequencing and transcriptomes to systematically investigate PPRs in Salvia miltiorrhiza, which is a well-known material of traditional Chinese medicine and an emerging model system for medicinal plant studies. Among 562 identified SmPPRs, 299 belong to the P subfamily while the others belong to the PLS subfamily. The majority of SmPPRs have only one exon and are localized in the mitochondrion or chloroplast. As many as 546 SmPPRs were expressed in at least one tissue and exhibited differential expression patterns, which indicates they likely play a variety of functions in S. miltiorrhiza. Up to 349 SmPPRs were salicylic acid-responsive and 183 SmPPRs were yeast extract and Ag+-responsive, which indicates these genes might be involved in S. miltiorrhiza defense stresses and secondary metabolism. Furthermore, 23 salicylic acid-responsive SmPPRs were co-expressed with phenolic acid biosynthetic enzyme genes only while 16 yeast extract and Ag+-responsive SmPPRs were co-expressed with tanshinone biosynthetic enzyme genes only. Two SmPPRs were co-expressed with both phenolic acid and tanshinone biosynthetic enzyme genes. The results provide a useful platform for further investigating the roles of PPRs in S. miltiorrhiza.
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