Trityl-based EPR probe with enhanced sensitivity to oxygen.
Trityl-based EPR probe with enhanced sensitivity to oxygen.
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DOI：
10.1016/j.freeradbiomed.2009.06.007
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发表时间：
2009-09-01
影响因子：
7.4
通讯作者：
Khramtsov, Valery V.
中科院分区：
文献类型：
--
作者：
Bobko, Andrey A.;Dhimitruka, Ilirian;Eubank, Timothy D.;Marsh, Clay B.;Zweier, Jay L.;Khramtsov, Valery V.
合成了一种含一个醛基和两个羧基的不对称三芳甲基衍生物TAM-H。TAM-H的电子顺磁共振（EPR）谱的特征在于在缺氧条件下具有105 mG的线宽和245 mG的超精细相互作用常数的窄线的双峰。的双峰的组件的部分重叠的结果在增强的灵敏度的光谱振幅比氧相比，氧引起的线宽加宽的一条线。TAM-H探头的应用允许在从大气压到1 mmHg的扩展氧气压力范围内进行EPR测量，而流行的基于TAM的氧气传感器Oxo 63的EPR光谱线宽实际上对低于20 mmHg的氧气分压不敏感。TAM-H探针相对于Oxo 63在检测Met-1癌细胞的氧消耗中表现出增强的灵敏度。TAM-H探头允许在缺氧阶段和真正缺氧（≤ 1.5 mmHg）期间延长氧耗测量。
An asymmetric derivative of triarylmethyl radical, TAM-H, containing one aldehyde and two carboxyl groups was synthesized. The electron paramagnetic resonance, EPR, spectrum of TAM-H is characterized by a doublet of narrow lines with linewidth of 105 mG in anoxic conditions and hyperfine interaction constant 245 mG. The partial overlap of the components of the doublet results in enhanced sensitivity of the spectral amplitudes ratio to oxygen compared with oxygen-induced linewidth broadening of a single line. Application of the TAM-H probe allows for EPR measurements in an extended range of oxygen pressures from atmospheric to 1 mmHg whereas the EPR spectrum linewidth of the popular TAM-based oxygen sensor, Oxo63, is practically insensitive to oxygen partial pressures below 20 mmHg. Enhanced sensitivity of TAM-H probe relative to Oxo63 was demonstrated in detection of oxygen consumption by Met-1 cancer cells. The TAM-H probe allowed prolonged measurements of oxygen depletion during the hypoxia stage and down to true anoxia (≤ 1.5 mmHg).
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DOI：
10.1152/ajpheart.00192.2003
发表时间：
2003-11-01
影响因子：
4.8
作者：
Marcinek, DJ;Ciesielski, WA;Schenkman, KA
通讯作者：
Schenkman, KA
影响因子：
7.4
作者：
Liu, Yangping;Villamena, Frederick A.;Sun, Jian;Wang, Tse-Yao;Zweier, Jay L.
通讯作者：
Zweier, Jay L.
影响因子：
3.3
作者：
Gallez, B;Bacic, G;Swartz, HM
通讯作者：
Swartz, HM
影响因子：
3.3
作者：
GODA, F;LIU, KJ;SWARTZ, HM
通讯作者：
SWARTZ, HM
DOI：
10.1073/pnas.042671399
发表时间：
2002-02-19
影响因子：
11.1
作者：
Krishna, MC;English, S;Mitchell, JB
通讯作者：
Mitchell, JB