Paratransgenesis: a promising new strategy for mosquito vector control.
Paratransgenesis: a promising new strategy for mosquito vector control.
复制标题
DOI：
10.1186/s13071-015-0959-2
复制
发表时间：
2015-06-24
影响因子：
3.2
通讯作者：
Marrelli MT
中科院分区：
文献类型：
--
作者：
Wilke AB;Marrelli MT
按蚊、伊蚊和库蚊这三个主要蚊属分别传播疟疾、登革热和淋巴丝虫病。目前的蚊子控制策略被证明是不成功的，这些疾病仍然有相当数量的发病率和死亡率。基因控制方法现已作为有希望的替代策略出现，基于两种方法：用耐病蚊子取代媒介种群，以及释放携带致命基因的蚊子以抑制目标种群。然而，如果要成功地使用这些方法，就需要克服大量的障碍和限制，最重要的是，每个目标物种都需要一个转基因蚊子品系，这使得当同一地区有多个媒介蚊子时，转基因蚊子策略是不可行的。能够在多种蚊子物种上定居的转基因细菌可能是解决这一问题的办法，也是控制这些疾病的另一种选择。在副转基因方法中，共生菌被转基因并重新引入蚊子体内，在那里它们表达效应器分子。然而，要将这种方法用于实践，需要更好地了解蚊子的微生物区系，并识别共生细菌和效应器分子。事实证明，在天生难以转化的蚊子物种或兄弟物种复合体中，副转基因非常有用。在这种方法中，转基因细菌可以通过以下方式发挥作用：(A)在宿主中引起致病效应；(B)干扰宿主的繁殖；(C)降低载体的能力；以及(D)干扰卵子发生和胚胎发生。这是一种比使用转基因蚊子更灵活和适应性更强的方法，因为如果效应器分子和共生菌没有达到预期的结果，它们可以被替换。因此，副转基因可能成为蚊子控制的重要综合有害生物管理工具。
The three main mosquito genera, Anopheles, Aedes and Culex, transmit respectively malaria, dengue and lymphatic filariasis. Current mosquito control strategies have proved unsuccessful, and there still is a substantial number of morbidity and mortality from these diseases. Genetic control methods have now arisen as promising alternative strategies, based on two approaches: the replacement of a vector population by disease-refractory mosquitoes and the release of mosquitoes carrying a lethal gene to suppress target populations. However, substantial hurdles and limitations need to be overcome if these methods are to be used successfully, the most significant being that a transgenic mosquito strain is required for every target species, making genetically modified mosquito strategies inviable when there are multiple vector mosquitoes in the same area. Genetically modified bacteria capable of colonizing a wide range of mosquito species may be a solution to this problem and another option for the control of these diseases. In the paratransgenic approach, symbiotic bacteria are genetically modified and reintroduced in mosquitoes, where they express effector molecules. For this approach to be used in practice, however, requires a better understanding of mosquito microbiota and that symbiotic bacteria and effector molecules be identified. Paratransgenesis could prove very useful in mosquito species that are inherently difficult to transform or in sibling species complexes. In this approach, a genetic modified bacteria can act by: (a) causing pathogenic effects in the host; (b) interfering with the host’s reproduction; (c) reducing the vector’s competence; and (d) interfering with oogenesis and embryogenesis. It is a much more flexible and adaptable approach than the use of genetically modified mosquitoes because effector molecules and symbiotic bacteria can be replaced if they do not achieve the desired result. Paratransgenesis may therefore become an important integrated pest management tool for mosquito control.
登录
查看更多内容
影响因子：
2.7
作者：
Chavshin, Ali Reza;Oshaghi, Mohammad Ali;Ghoorchian, Sadigheh
通讯作者：
Ghoorchian, Sadigheh
影响因子：
1.9
作者：
Allen, ML;Christensen, BM
通讯作者：
Christensen, BM
Wolbachia菌株WPIP产生了一种细胞质不相容性的模式，从而增强了针对疾病媒介艾德斯白化病的基于Wolbachia的抑制策略。
影响因子：
3.2
作者：
Calvitti M;Moretti R;Skidmore AR;Dobson SL
通讯作者：
Dobson SL
影响因子：
64.8
作者：
Catteruccia, F;Nolan, T;Crisanti, A
通讯作者：
Crisanti, A
影响因子：
1.9
作者：
Coutinho-Abreu IV;Zhu KY;Ramalho-Ortigao M
通讯作者：
Ramalho-Ortigao M