Novel Functional Analysis for Pathogenic Proteins of Bursaphelenchus xylophilus in Pine Seed Embryos Using a Virus Vector.
Novel Functional Analysis for Pathogenic Proteins of Bursaphelenchus xylophilus in Pine Seed Embryos Using a Virus Vector.
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使用病毒载体在松树种子胚胎中的Bursaphelenchus xylophilus的致病蛋白进行新的功能分析。
DOI：
10.3389/fpls.2022.872076
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
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由松材线虫引起的松树枯萎病是世界范围内危害最严重的林木病害之一。PWD被认为是由对B的连续过度过敏反应引发的。木生植物先前的研究已经报道了在对B敏感的松树中诱导过敏反应的候选致病分子。木生植物其中一些分子的功能已经在模式植物中使用瞬时过表达进行了分析，然而，由于缺乏合适的功能分析方法，它们是否可以在天然宿主松树中诱导过敏反应仍不清楚。本研究利用苹果潜伏球形病毒载体瞬时过表达技术，建立了一种新的感病黑松种胚功能分析方法。木霉菌引起烟草细胞死亡，以确定它们是否诱导松树过敏反应。我们发现五种分子中的三种诱导了发病相关基因的显著更高表达（p < 0.05），表明与模拟和绿色荧光蛋白对照相比，松树种子胚中的过敏反应。这一结果表明，基于烟草的筛查可能会检测到假阳性。本研究首次对B致病候选分子的功能进行了分析。利用外源基因表达技术在天然寄主松树中表达松材线虫，有望成为研究松材线虫病发病机制的有力工具。
Pine wilt disease (PWD), which is caused by the pine wood nematode Bursaphelenchus xylophilus, is among the most serious tree diseases worldwide. PWD is thought to be initiated by sequential excessive hypersensitive responses to B. xylophilus. Previous studies have reported candidate pathogenic molecules inducing hypersensitive responses in pine trees susceptible to B. xylophilus. The functions of some of these molecules have been analyzed in model plants using transient overexpression; however, whether they can induce hypersensitive responses in natural host pines remains unclear due to the lack of a suitable functional analysis method. In this study, we established a novel functional analysis method for susceptible black pine (Pinus thunbergii) seed embryos using transient overexpression by the Apple latent spherical virus vector and investigated five secreted proteins of B. xylophilus causing cell death in tobacco to determine whether they induce hypersensitive responses in pine. We found that three of five molecules induced significantly higher expression in pathogenesis-related genes ( p < 0.05), indicating hypersensitive response in pine seed embryos compared with mock and green fluorescence protein controls. This result suggests that tobacco-based screening may detect false positives. This study is the first to analyze the function of pathogenic candidate molecules of B. xylophilus in natural host pines using exogenous gene expression, which is anticipated to be a powerful tool for investigating the PWD mechanism.
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影响因子：
3.7
作者：
Shinya R;Morisaka H;Kikuchi T;Takeuchi Y;Ueda M;Futai K
通讯作者：
Futai K
影响因子：
6.7
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Kikuchi T;Cotton JA;Dalzell JJ;Hasegawa K;Kanzaki N;McVeigh P;Takanashi T;Tsai IJ;Assefa SA;Cock PJ;Otto TD;Hunt M;Reid AJ;Sanchez-Flores A;Tsuchihara K;Yokoi T;Larsson MC;Miwa J;Maule AG;Sahashi N;Jones JT;Berriman M
通讯作者：
Berriman M
影响因子：
3.7
作者：
Ding X;Ye J;Lin S;Wu X;Li D;Nian B
通讯作者：
Nian B
影响因子：
4.4
作者：
Espada M;Eves-van den Akker S;Maier T;Vijayapalani P;Baum T;Mota M;Jones JT
通讯作者：
Jones JT
影响因子：
3.7
作者：
Huang, Xin;Hu, Longjiao;Wu, Xiaoqin
通讯作者：
Wu, Xiaoqin