Large-scale screening of natural genetic resource in the hydrocarbon-producing microalga Botrycoccus braunii identified novel fast-growing strains.
Large-scale screening of natural genetic resource in the hydrocarbon-producing microalga Botrycoccus braunii identified novel fast-growing strains.
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DOI：
10.1038/s41598-021-86760-8
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发表时间：
2021-04-02
影响因子：
4.6
通讯作者：
Okada S
中科院分区：
文献类型：
--
作者：
Kawamura K;Nishikawa S;Hirano K;Ardianor A;Nugroho RA;Okada S
藻类生物燃料研究旨在通过使用产油微藻制造可再生、碳中和的生物燃料。淡水微藻布朗葡萄球菌（Botryococcus braunii）由于其能够积累大量石油类烃但生长缓慢而受到广泛关注。我们进行了大规模的快速增长的菌株与180株分离自22个池塘位于从热带到冷温带的广泛的地理范围内的筛选。一个快速增长的菌株，昭和，其中记录了最高的藻类碳氢化合物的生产力，到目前为止，被用作基准。通过监测玻璃管中的光密度进行初步筛选，并鉴定出9种生长速率比Showa更快或相当的野生菌株。基于生物量的评估表明，这些菌株的生物量和烃生产率分别比昭和高12-37%和11-88%。其中一株OIT-678以1.2天的倍增时间创造了B小种菌株中生长速度最快的新纪录。在相同的培养条件下，OIT-678的生物质生产率比Showa高36%，碳氢化合物生产率高34%，生物质密度高20%，这表明新菌株有可能打破碳氢化合物最高生产率的记录。
Algal biofuel research aims to make a renewable, carbon–neutral biofuel by using oil-producing microalgae. The freshwater microalga Botryococcus braunii has received much attention due to its ability to accumulate large amounts of petroleum-like hydrocarbons but suffers from slow growth. We performed a large-scale screening of fast-growing strains with 180 strains isolated from 22 ponds located in a wide geographic range from the tropics to cool-temperate. A fast-growing strain, Showa, which recorded the highest productivities of algal hydrocarbons to date, was used as a benchmark. The initial screening was performed by monitoring optical densities in glass tubes and identified 9 wild strains with faster or equivalent growth rates to Showa. The biomass-based assessments showed that biomass and hydrocarbon productivities of these strains were 12–37% and 11–88% higher than that of Showa, respectively. One strain, OIT-678 established a new record of the fastest growth rate in the race B strains with a doubling time of 1.2 days. The OIT-678 had 36% higher biomass productivity, 34% higher hydrocarbon productivity, and 20% higher biomass density than Showa at the same cultivation conditions, suggesting the potential of the new strain to break the record for the highest productivities of hydrocarbons.
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影响因子：
3.3
作者：
Eroglu, Ela;Okada, Shigeru;Melis, Anastasios
通讯作者：
Melis, Anastasios
影响因子：
15.9
作者：
Banerjee, Sanjukta;Banerjee, Srijoni;Das, Debabrata
通讯作者：
Das, Debabrata
影响因子：
3.8
作者：
CASADEVALL, E;DIF, D;DESANTI, O
通讯作者：
DESANTI, O
影响因子：
11.4
作者：
Ho, Shih-Hsin;Chen, Chun-Yen;Chang, Jo-Shu
通讯作者：
Chang, Jo-Shu
DOI：
10.1016/j.algal.2012.05.003
发表时间：
2012-10-01
影响因子：
5.1
作者：
Kawachi, Masanobu;Tanoi, Takako;Watanabe, Makoto M.
通讯作者：
Watanabe, Makoto M.