LeafGo: Leaf to Genome, a quick workflow to produce high-quality de novo plant genomes using long-read sequencing technology.
LeafGo: Leaf to Genome, a quick workflow to produce high-quality de novo plant genomes using long-read sequencing technology.
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Leafgo:Leaf至Genome,一种快速的工作流程,使用长阅读测序技术产生高质量的从头植物基因组。
DOI:
10.1186/s13059-021-02475-z
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发表时间:
2021-09-03
期刊:
影响因子:
12.3
通讯作者:
Ermini L
中科院分区:
文献类型:
--
作者:
Driguez P;Bougouffa S;Carty K;Putra A;Jabbari K;Reddy M;Soppe R;Cheung MS;Fukasawa Y;Ermini L
Currently, different sequencing platforms are used to generate plant genomes and no workflow has been properly developed to optimize time, cost, and assembly quality. We present LeafGo, a complete de novo plant genome workflow, that starts from tissue and produces genomes with modest laboratory and bioinformatic resources in approximately 7 days and using one long-read sequencing technology. LeafGo is optimized with ten different plant species, three of which are used to generate high-quality chromosome-level assemblies without any scaffolding technologies. Finally, we report the diploid genomes of Eucalyptus rudis and E. camaldulensis and the allotetraploid genome of Arachis hypogaea. The online version contains supplementary material available at 10.1186/s13059-021-02475-z.
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