Effect of dietary supplementation of fish oil for lactating sows and weaned piglets on piglet Th polarization
Effect of dietary supplementation of fish oil for lactating sows and weaned piglets on piglet Th polarization
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哺乳母猪和断奶仔猪日粮添加鱼油对仔猪Th极化的影响
DOI：
10.1016/j.livsci.2009.08.002
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发表时间：
2009-12
影响因子：
1.8
通讯作者：
Huang, F. R.
中科院分区：
文献类型：
--
作者：
Jiang, S. W.;Xiao, C. L.;Chen, W.;Luo, J.;Peng, J.;Huang, F. R.
本研究旨在探讨日粮中添加鱼油对仔猪辅助性 T 细胞 (Th) 极化的影响，以及其对仔猪血清干扰素 γ (IFN-γ) 和白细胞介素 10 (IL-10) 浓度以及脾脏 Th1/Th2 特征基因表达的影响。 18 头妊娠母猪从分娩前 10 d 至断奶期间日粮中添加 7% 猪油 (C) (n=10) 或 7% 鱼油 (T) (n=8)。断奶时，设计了裂区试验，将56头仔猪，每组28头，来自饲喂鱼油日粮或猪油日粮的母猪，根据哺乳期母猪日粮和断奶后仔猪日粮（C为7%猪油，T为7%鱼油）分为四组，每组7个重复（每个重复1雌1公）：CC、CT、TC、TT，饲喂7%鱼油或猪油日粮。第35天至第70天。测量并分析脾脏中IFN-γ和IL-10的血清浓度以及Th1/Th2相关基因的表达水平。结果表明，断奶后鱼油饲料喂养的仔猪血清IFN-γ/IL-10比值往往高于猪油饲料喂养的仔猪（P=0.09）。哺乳期鱼油饲喂增加脾脏IL-12b、IL-12受体β2（IL-12Rβ2）、IL-2和IFN-γ基因表达（P<0.05或P<0.01），断奶后饲喂鱼油增加脾脏IL-12b（P=0.06）、IL-2（P<0.01）和IFN-γ（P=0.08）mRNA表达量实验结束时用猪油饲料喂养仔猪。另一方面，饲喂鱼油饲料的母猪的断奶仔猪脾脏中IL-4基因的表达量低于饲喂猪油饲料的母猪的断奶仔猪。然而，饲喂鱼油饲料的断奶仔猪脾脏中IL-4（P=0.06）、IL-6（P<0.01）和IL-10（P=0.05）mRNA丰度高于猪油饲喂仔猪。这些结果表明，哺乳期膳食鱼油可以增加 Th1 极化并加速免疫成熟；而断奶仔猪日粮中添加7%鱼油可能会增加Th2细胞因子的表达。
The present study was designed to investigate the effect of dietary fish oil supplementation on piglet T helper cells (Th) polarization in relation to its impact on piglet serum interferon γ (IFN-γ) and interleukin 10 (IL-10) concentrations and splenic expression of Th1/Th2 characteristic genes. The diets of 18 gestating sows were supplemented with 7% lard (C) (n=10) or 7% fish oil (T) (n=8) from 10 d before parturition to weaning. At weaning, a split plot experiment was designed, 56 piglets, 28 each from sows fed with fish oil diet or lard diet, were divided into four groups of 7 replicates (one female and one castrated male per replicate) based on both sow diet during lactation and post-weaning piglet diet (C had 7% lard and T had 7% fish oil): CC, CT, TC, TT, and were fed the 7% fish oil or lard diet from day 35 to day 70. Serum concentrations of IFN-γ and IL-10, and Th1/Th2 related genes expression levels in spleen were measured and analyzed. The results showed that piglets fed with fish oil diet during post-weaning tended to have higher serum IFN-γ/IL-10 ratio (P=0.09) than lard diet fed piglets. Lactation fish oil feeding increased splenic IL-12b, IL-12 receptor β2 (IL-12Rβ2), IL-2 and IFN-γ genes expression (P<0.05 or P<0.01) and post-weaning fish oil feeding increased splenic IL-12b (P=0.06), IL-2 (P<0.01) and IFN-γ (P=0.08) mRNA expression than that in lard diet fed piglets at the end of this experiment. On the other hand, IL-4 gene expression (P=0.01) in spleen was lower in weaned piglet from fish oil diet fed sows than that from lard diet fed sows. However, post-weaning piglets fed fish oil diet had higher splenic IL-4 (P=0.06), IL-6 (P<0.01) and IL-10 (P=0.05) mRNA abundances than that fed with lard diet. These results indicated that dietary fish oil during lactation could increase Th1 polarization and accelerate immune maturation; while 7% fish oil in weaned piglets' diet was likely to increase Th2 cytokines expression.
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影响因子：
4.2
作者：
MEYDANI, SN;ENDRES, S;GORBACH, SL
通讯作者：
GORBACH, SL
影响因子：
56.9
作者：
SMITH, KA
通讯作者：
SMITH, KA
影响因子：
5.5
作者：
F. Wallace;E. Miles;Carl Evans;Theresa E. Stock;P. Yaqoob;P. Calder
通讯作者：
F. Wallace;E. Miles;Carl Evans;Theresa E. Stock;P. Yaqoob;P. Calder
影响因子：
7.1
作者：
Caughey, GE;Mantzioris, E;James, MJ
通讯作者：
James, MJ
影响因子：
1.9
作者：
Lauritzen, L;Kjær, TMR;Frokiær, H
通讯作者：
Frokiær, H