Expression and purification of an influenza hemagglutinin--one step closer to a recombinant protein-based influenza vaccine.
Expression and purification of an influenza hemagglutinin--one step closer to a recombinant protein-based influenza vaccine.
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DOI：
10.1016/j.vaccine.2005.11.005
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发表时间：
2006-03-15
期刊：
影响因子：
5.5
通讯作者：
Cox MM
中科院分区：
文献类型：
--
作者：
Wang K;Holtz KM;Anderson K;Chubet R;Mahmoud W;Cox MM
近年来，亚洲大量人感染禽流感病毒，这引发了人们对下一次流感大流行即将到来的担忧。对抗流感最有效的方法是为公众接种疫苗。然而，使用传统的鸡蛋疫苗方法开发流感疫苗至少需要3-6个月。流感血凝素蛋白(HA)是目前疫苗中的活性成分，利用杆状病毒表达载体系统可在昆虫细胞中表达并快速纯化。基于这种重组抗原的流感疫苗可以更及时地应对潜在的流感大流行。在这里，我们报道了一种创新的重组HA(RHA)表达监测方法和一种快速纯化过程。各种生化分析表明，纯化的RHA折叠正确，具有生物活性。
Numerous human infections with avian influenza viruses in Asia in recent years have raised the concern that the next influenza pandemic is imminent. The most effective way to combat influenza is through the vaccination of the public. However, a minimum of 3–6 months is needed to develop an influenza vaccine using the traditional egg-based vaccine approach. The influenza hemagglutinin protein (HA), the active ingredient in the current vaccine, can be expressed in insect cells using the baculovirus expression vector system and purified rapidly. An influenza vaccine based on such a recombinant antigen allows a more timely response to a potential influenza pandemic. Here, we report an innovative monitoring assay for recombinant HA (rHA) expression and a rapid purification process. Various biochemical analyses indicate that the purified rHA is properly folded and biologically active.
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